5NSR
Cryo-EM structure of RNA polymerase-sigma54 holo enzyme with promoter DNA closed complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | M | RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor | polymer | 573 | 62158.8 | 1 | UniProt (A0A0J4U551) Pfam (PF00309) Pfam (PF04963) Pfam (PF04552) UniProt (by SIFTS) (P06223) In PDB | Klebsiella pneumoniae | |
| 6 | F | Non-Template promoter DNA | polymer | 63 | 19404.4 | 1 | Klebsiella pneumoniae | ||
| 7 | G | Template DNA promoter | polymer | 63 | 19439.4 | 1 | Klebsiella pneumoniae |
Sequence modifications
M: -101 - 15 (UniProt: A0A0J4U551)
M: 16 - 49 (PDB: 5NSR)
M: 118 - 258 (UniProt: A0A0J4U551)
M: 259 - 304 (PDB: 5NSR)
M: 306 - 396 (UniProt: A0A0J4U551)
M: 398 - 413 (PDB: 5NSR)
M: 415 - 477 (UniProt: A0A0J4U551)
M: 16 - 49 (PDB: 5NSR)
M: 118 - 258 (UniProt: A0A0J4U551)
M: 259 - 304 (PDB: 5NSR)
M: 306 - 396 (UniProt: A0A0J4U551)
M: 398 - 413 (PDB: 5NSR)
M: 415 - 477 (UniProt: A0A0J4U551)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 490557.2 | |
| All* | Total formula weight | 490557.2 |
