5NRO

Structure of full-length DnaK with bound J-domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Chaperone protein DnaK	polymer	611	66393.9	1	UniProt (P0A6Y8) Pfam (PF00012)	Escherichia coli	HSP70,Heat shock 70 kDa protein,Heat shock protein 70
2	B (B)	Chaperone protein DnaJ	polymer	108	11918.1	1	UniProt (P08622) Pfam (PF00226)	Escherichia coli	HSP40,Heat shock protein J
3	C (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
4	D (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	E (A)	water	water		18.0	4	Chemie (HOH)

Sequence modifications

A: 1 - 605 (UniProt: P0A6Y8)

PDB	External Database	Details
Cys 47	Glu 47	engineered mutation
Ala 199	Thr 199	engineered mutation
Cys 529	Phe 529	engineered mutation
His 606	-	expression tag
His 607	-	expression tag
His 608	-	expression tag
His 609	-	expression tag
His 610	-	expression tag
His 611	-	expression tag

B: 1 - 105 (UniProt: P08622)

PDB	External Database	Details
Ser 106	-	expression tag
Ser 107	-	expression tag
His 108	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	78312.0
Non-Polymers*	Number of molecules	2
	Total formula weight	531.5
All*	Total formula weight	78843.5

*Water molecules are not included.