5NQV

Structure of the Arabidopsis Thaliana TOPLESS N-terminal domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Protein TOPLESS	polymer	210	25292.8	4	UniProt (Q94AI7) Pfam (PF21359) In PDB	Arabidopsis thaliana (Mouse-ear cress)	WUS-interacting protein 1
2	E, F, G, H	EAR motif of IAA27	polymer	11	1172.3	4	UniProt (by SIFTS) (Q9ZSY8) In PDB	synthetic construct
3	A, C, D	L(+)-TARTARIC ACID	non-polymer		150.1	4	Chemie (TLA)
4	B, C, E	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
5		water	water		18.0	530	Chemie (HOH)

Sequence modifications

A, B, C, D: 3 - 184 (UniProt: Q94AI7)

PDB	External Database	Details
Met -25	-	initiating methionine
Lys -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Pro -17	-	expression tag
Met -16	-	expression tag
Ser -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Met 1	-	expression tag
Gly 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	105860.5
Non-Polymers*	Number of molecules	10
	Total formula weight	1152.9
All*	Total formula weight	107013.4

*Water molecules are not included.