5NQK

human 199.16 TCR in complex with Melan-A/MART-1 (26-35) peptide and HLA-A2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	H	HLA class I histocompatibility antigen, A-2 alpha chain	polymer	276	31979.4	1	UniProt (P01892) Pfam (PF00129) Pfam (PF07654) UniProt (by SIFTS) (P04439) In PDB	Homo sapiens (Human)	MHC class I antigen A*2
2	L	Beta-2-microglobulin	polymer	100	11879.4	1	UniProt (P61769) Pfam (PF07654) In PDB	Homo sapiens (Human)
3	P	Melanoma antigen recognized by T-cells 1	polymer	10	985.2	1	UniProt (by SIFTS) (Q16655) In PDB	Homo sapiens	MART-1,ANTIGEN LB39-AA,ANTIGEN SK29-AA,PROTEIN MELAN-A, MELAN-A/MART-1 (26-35) DECAPEPTIDE
4	A	T-cell receptor alpha variable 12-2,T-cell receptor alpha joining 45,T-cell receptor alpha chain C region	polymer	211	23090.3	1	UniProt (A0A075B6T6) UniProt (A0A075B6X0) UniProt (A0A1B0GUM0) Pfam (PF09291) UniProt (by SIFTS) (P01848) In PDB	Homo sapiens (Human)	199.54 TCR ALPHA CHAIN (TRAJ 12-2, TRAJ 45),T-CELL RECEPTOR, 199.54 TCR ALPHA CHAIN (TRAJ 12-2, TRAJ 45)
5	B	T-cell receptor beta variable 19,TRB protein	polymer	251	28292.4	1	UniProt (A0A5B3) UniProt (A0A0C4ZKA8) Pfam (PF07686) UniProt (by SIFTS) (A0A075B6N1) In PDB	Homo sapiens (Human)	V_SEGMENT TRANSLATION PRODUCT,199.16 TCR BETA CHAIN (TRBV 19, TRBD2, TRBJ 2-2, TRBC2),199.16 TCR BETA CHAIN (TRBV 19, TRBD2, TRBJ 2-2, TRBC2)
6	B	SODIUM ION	non-polymer		23.0	1	Chemie (NA)

Sequence modifications

H: 1 - 276 (UniProt: P01892)

PDB	External Database	Details
Val 245	Ala 269	conflict

L: 1 - 99 (UniProt: P61769)

PDB	External Database	Details
Met 0	-	initiating methionine

A: -1 - 91 (UniProt: A0A075B6T6)

PDB	External Database	Details
Met -1	Ser 20	initiating methionine
Ser 49	Phe 70	conflict
Gly 91	Val 112	conflict

A: 92 - 108 (UniProt: A0A075B6X0)
A: 109 - 202 (UniProt: A0A1B0GUM0)

PDB	External Database	Details
Cys 158	Thr 160	engineered mutation
Asn 203	-	expression tag
Asp 204	-	expression tag
Gly 205	-	expression tag
Gly 206	-	expression tag
Gly 207	-	expression tag
Cys 208	-	expression tag
Lys 209	-	expression tag

B: 3 - 94 (UniProt: A0A5B3)

PDB	External Database	Details
Met 2	-	initiating methionine
Gln 95	-	linker
Gly 96	-	linker
Leu 97	-	linker
Ala 98	-	linker
Gly 99	-	linker
Ala 100	-	linker

B: 101 - 244 (UniProt: A0A0C4ZKA8)

PDB	External Database	Details
Cys 171	Ser 101	conflict
Gln 245	-	expression tag
Asp 246	-	expression tag
Arg 247	-	expression tag
Gly 248	-	expression tag
Gly 249	-	expression tag
Gly 250	-	expression tag
Cys 251	-	expression tag
Asp 252	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	96226.6
Non-Polymers*	Number of molecules	1
	Total formula weight	23.0
All*	Total formula weight	96249.6

*Water molecules are not included.