5NPO

Promiscuous Protein Self-Assembly as a Function of Protein Stability

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Beta-lactamase TEM	polymer	264	29087.2	1	UniProt (P62593) Pfam (PF00144) Pfam (PF13354) In PDB	Escherichia coli	IRT-4,Penicillinase,TEM-1,TEM-16/CAZ-7,TEM-2,TEM-24/CAZ-6,TEM-3,TEM-4,TEM-5,TEM-6,TEM-8/CAZ-2
2	B	Beta-lactamase	polymer	265	28955.1	1	UniProt (D3INY1) Pfam (PF00144) Pfam (PF13354) In PDB	Escherichia coli
3	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4		water	water		18.0	38	Chemie (HOH)

Sequence modifications

A: 26 - 288 (UniProt: P62593)

PDB	External Database	Details
Met 25	-	initiating methionine
Ile 84	Val 82	conflict

B: 25 - 288 (UniProt: D3INY1)

PDB	External Database	Details
Lys 37	Glu 35	engineered mutation
Lys 42	Ala 40	engineered mutation
Ala 52	Asn 50	engineered mutation
Val 58	Glu 56	engineered mutation
Gly 120	Arg 118	engineered mutation
Val 184	Ala 182	engineered mutation
Ala 201	Leu 199	engineered mutation
Met 263	Thr 261	engineered mutation
Ala 289	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58042.4
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	58066.7

*Water molecules are not included.