5NPO
Promiscuous Protein Self-Assembly as a Function of Protein Stability
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Beta-lactamase TEM | polymer | 264 | 29087.2 | 1 | UniProt (P62593) Pfam (PF00144) Pfam (PF13354) | Escherichia coli | IRT-4,Penicillinase,TEM-1,TEM-16/CAZ-7,TEM-2,TEM-24/CAZ-6,TEM-3,TEM-4,TEM-5,TEM-6,TEM-8/CAZ-2 |
| 2 | B (B) | Beta-lactamase | polymer | 265 | 28955.1 | 1 | UniProt (D3INY1) Pfam (PF00144) Pfam (PF13354) | Escherichia coli | |
| 3 | C (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 4 | D, E (A, B) | water | water | 18.0 | 38 | Chemie (HOH) |
Sequence modifications
A: 26 - 288 (UniProt: P62593)
B: 25 - 288 (UniProt: D3INY1)
| PDB | External Database | Details |
|---|---|---|
| Met 25 | - | initiating methionine |
| Ile 84 | Val 82 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Lys 37 | Glu 35 | engineered mutation |
| Lys 42 | Ala 40 | engineered mutation |
| Ala 52 | Asn 50 | engineered mutation |
| Val 58 | Glu 56 | engineered mutation |
| Gly 120 | Arg 118 | engineered mutation |
| Val 184 | Ala 182 | engineered mutation |
| Ala 201 | Leu 199 | engineered mutation |
| Met 263 | Thr 261 | engineered mutation |
| Ala 289 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 58042.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 24.3 | |
| All* | Total formula weight | 58066.7 |
