5NJC

E. coli Microcin-processing metalloprotease TldD/E (TldD E263A mutant) with hexapeptide bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Metalloprotease TldD	polymer	495	53033.5	2	UniProt (P0AGG8) Pfam (PF01523) Pfam (PF19290) Pfam (PF19289) In PDB	Escherichia coli str. K-12 substr. MG1655
2	B, D	Metalloprotease PmbA	polymer	450	48421.5	2	UniProt (P0AFK0) Pfam (PF01523) Pfam (PF19290) Pfam (PF19289) In PDB	Escherichia coli str. K-12 substr. MG1655	Protein TldE
3	E, F	VAL-LEU-GLU-ASP-ARG-ILE	polymer	6	744.9	2		Escherichia coli str. K-12 substr. MG1655
4	A, C	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	A, B, C, D	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	4	Chemie (MES)
6	A, B, C, D	1,2-ETHANEDIOL	non-polymer		62.1	10	Chemie (EDO)
7	A, B, C, D	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
8		water	water		18.0	2086	Chemie (HOH)

Sequence modifications

A, C: 1 - 481 (UniProt: P0AGG8)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Ala 263	Glu 263	engineered mutation
Asp 401	Gly 401	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	204399.7
Non-Polymers*	Number of molecules	20
	Total formula weight	1624.4
All*	Total formula weight	206024.1

*Water molecules are not included.