5NJB

E. coli Microcin-processing metalloprotease TldD/E with actinonin bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Metalloprotease TldD	polymer	495	53091.6	2	UniProt (P0AGG8) Pfam (PF01523) Pfam (PF19290) Pfam (PF19289)	Escherichia coli K-12
2	B, D (B, D)	Metalloprotease PmbA	polymer	450	48421.5	2	UniProt (P0AFK0) Pfam (PF01523) Pfam (PF19290) Pfam (PF19289)	Escherichia coli K-12	Protein TldE
3	E, L (A, C)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	F, M (A, C)	ACTINONIN	non-polymer		385.5	2	Chemie (BB2)
5	G, N (A, C)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
6	H, I, J, K, O... (A, B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	9	Chemie (EDO)
7	T, U, V, W (A, B, C, D)	water	water		18.0	2124	Chemie (HOH)

Sequence modifications

A, C: 1 - 481 (UniProt: P0AGG8)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Asp 401	Gly 401	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	203026.0
Non-Polymers*	Number of molecules	15
	Total formula weight	1850.9
All*	Total formula weight	204876.9

*Water molecules are not included.