5NJ5

E. coli Microcin-processing metalloprotease TldD/E with phosphate bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Metalloprotease TldD	polymer	495	53091.6	2	UniProt (P0AGG8) Pfam (PF01523) Pfam (PF19290) Pfam (PF19289)	Escherichia coli str. K-12 substr. MG1655
2	B, D (B, D)	Metalloprotease PmbA	polymer	450	48421.5	2	UniProt (P0AFK0) Pfam (PF01523) Pfam (PF19290) Pfam (PF19289)	Escherichia coli str. K-12 substr. MG1655	Protein TldE
3	E, M (A, C)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	F, N (A, C)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
5	G, H, I, J, K... (A, B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	12	Chemie (EDO)
6	U, V, W, X (A, B, C, D)	water	water		18.0	1797	Chemie (HOH)

Sequence modifications

A, C: 1 - 481 (UniProt: P0AGG8)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Asp 401	Gly 401	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	203026.0
Non-Polymers*	Number of molecules	16
	Total formula weight	1065.6
All*	Total formula weight	204091.6

*Water molecules are not included.