5NIT
Glucose oxidase mutant A2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Glucose oxidase | polymer | 581 | 63081.8 | 1 | UniProt (P13006) Pfam (PF00732) Pfam (PF05199) | Aspergillus niger | Beta-D-glucose:oxygen 1-oxido-reductase,Glucose oxyhydrase,GOD |
| 2 | B (B) | alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G22573RC) | |||
| 3 | C (A) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 1 | Chemie (FAD) | |||
| 4 | D (A) | OXYGEN MOLECULE | non-polymer | 32.0 | 1 | Chemie (OXY) | |||
| 5 | E, F, G (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 3 | Chemie (NAG) | |||
| 6 | H, I, J, K, L... (A) | 1,4-DIETHYLENE DIOXIDE | non-polymer | 88.1 | 18 | Chemie (DIO) | |||
| 7 | Z (A) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 1 | Chemie (EPE) | |||
| 8 | AA (A) | water | water | 18.0 | 427 | Chemie (HOH) |
Sequence modifications
A: 3 - 583 (UniProt: P13006)
| PDB | External Database | Details |
|---|---|---|
| Val 30 | Thr 52 | engineered mutation |
| Val 94 | Ile 116 | engineered mutation |
| Thr 162 | Ala 184 | engineered mutation |
| Lys 537 | Arg 559 | engineered mutation |
| Val 556 | Met 578 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 63081.8 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 748.7 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 3305.4 | |
| All* | Total formula weight | 67135.8 |
