5NIK

Structure of the MacAB-TolC ABC-type tripartite multidrug efflux pump

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Outer membrane protein TolC	polymer	479	52506.5	3	UniProt (P02930) Pfam (PF02321) In PDB	Escherichia coli (strain K12)	Multidrug efflux pump subunit TolC,Outer membrane factor TolC
2	D, E, F, G, H...	Macrolide export protein MacA	polymer	371	40715.7	6	UniProt (P75830) Pfam (PF16576) In PDB	Escherichia coli (strain K12)
3	J, K	Macrolide export ATP-binding/permease protein MacB	polymer	654	71600.1	2	UniProt (P75831) Pfam (PF00005) Pfam (PF12704) Pfam (PF02687) In PDB	Escherichia coli (strain K12)

Sequence modifications

A, B, C: 1 - 471 (UniProt: P02930)

PDB	External Database	Details
Leu 169	Val 191	conflict
Asp 472	-	expression tag
Tyr 473	-	expression tag
Lys 474	-	expression tag
Asp 475	-	expression tag
Asp 476	-	expression tag
Asp 477	-	expression tag
Asp 478	-	expression tag
Lys 479	-	expression tag

D, E, F, G, H, I: 1 - 371 (UniProt: P75830)

PDB	External Database	Details
Gln 139	Lys 139	conflict
Asn 148	Thr 148	conflict
Gln 251	Pro 251	conflict

J, K: 1 - 648 (UniProt: P75831)

PDB	External Database	Details
His 649	-	expression tag
His 650	-	expression tag
His 651	-	expression tag
His 652	-	expression tag
His 653	-	expression tag
His 654	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	545014.3
All*	Total formula weight	545014.3

*Water molecules are not included.