5NE5

Crystal structure of family 47 alpha-1,2-mannosidase from Caulobacter K31 strain in complex with kifunensine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Mannosyl-oligosaccharide 1,2-alpha-mannosidase	polymer	447	50546.7	1	UniProt (B0SWV2) Pfam (PF01532) In PDB	Caulobacter sp.
2	A	KIFUNENSINE	non-polymer		232.2	1	Chemie (KIF)
3	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	A	2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		209.2	1	Chemie (BTB)
5	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
6		water	water		18.0	418	Chemie (HOH)

Sequence modifications

A: 27 - 462 (UniProt: B0SWV2)

PDB	External Database	Details
Met 24	-	initiating methionine
Ala 25	-	expression tag
Ser 26	-	expression tag
Leu 463	-	expression tag
Glu 464	-	expression tag
His 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	50546.7
Non-Polymers*	Number of molecules	5
	Total formula weight	544.6
All*	Total formula weight	51091.3

*Water molecules are not included.