5NDX
The bacterial orthologue of Human a-L-iduronidase does not need N-glycan post-translational modifications to be catalytically competent: Crystallography and QM/MM insights into Mucopolysaccharidosis I
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Glycosyl hydrolase | polymer | 621 | 68879.1 | 1 | UniProt (A0A1B8R7L2) Pfam (PF01229) In PDB | Rhizobium leguminosarum bv. trifolii | Iduronidase |
| 2 | A | SULFATE ION | non-polymer | 96.1 | 11 | Chemie (SO4) | |||
| 3 | A | (2~{R},3~{S},4~{S},5~{R},6~{S})-6-(4-methyl-2-oxidanylidene-chromen-7-yl)oxy-3,4,5-tris(oxidanyl)oxane-2-carboxylic acid | non-polymer | 352.3 | 2 | Chemie (8U8) | |||
| 4 | water | water | 18.0 | 384 | Chemie (HOH) |
Sequence modifications
A: 1 - 621 (UniProt: A0A1B8R7L2)
| PDB | External Database | Details |
|---|---|---|
| Leu 65 | Val 65 | conflict |
| Gln 146 | Glu 146 | engineered mutation |
| Ser 312 | Asn 312 | conflict |
| Thr 355 | Ala 355 | conflict |
| Val 407 | Leu 407 | conflict |
| Ile 440 | Leu 440 | conflict |
| Ala 534 | Thr 534 | conflict |
| Ser 583 | Pro 583 | conflict |
| Glu 598 | Gln 598 | engineered mutation |
| Arg 607 | His 607 | conflict |
| Leu 618 | Val 618 | conflict |
| Ser 619 | Phe 619 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 68879.1 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1761.3 | |
| All* | Total formula weight | 70640.3 |
