5NDX
The bacterial orthologue of Human a-L-iduronidase does not need N-glycan post-translational modifications to be catalytically competent: Crystallography and QM/MM insights into Mucopolysaccharidosis I
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Glycosyl hydrolase | polymer | 621 | 68879.1 | 1 | UniProt (A0A1B8R7L2) | Rhizobium leguminosarum bv. trifolii | Iduronidase |
| 2 | B, C, D, E, F... (A) | SULFATE ION | non-polymer | 96.1 | 11 | Chemie (SO4) | |||
| 3 | M, N (A) | (2~{R},3~{S},4~{S},5~{R},6~{S})-6-(4-methyl-2-oxidanylidene-chromen-7-yl)oxy-3,4,5-tris(oxidanyl)oxane-2-carboxylic acid | non-polymer | 352.3 | 2 | Chemie (8U8) PubChem (25796464) PubChem (26470446) PubChem (91553) PubChem (24802323) PubChem (128746) PubChem (71433971) PubChem (71750690) PubChem (2733792) PubChem (1201526) PubChem (87296333) PubChem (14427077) PubChem (3683393) PubChem (95555420) PubChem (124489957) PubChem (7282394) PubChem (93982610) PubChem (7058243) PubChem (7282390) PubChem (7282392) PubChem (9928422) PubChem (124301865) PubChem (169447502) PubChem (127239645) PubChem (134813506) PubChem (134867435) PubChem (171395874) | |||
| 4 | O (A) | water | water | 18.0 | 384 | Chemie (HOH) |
Sequence modifications
A: 1 - 621 (UniProt: A0A1B8R7L2)
| PDB | External Database | Details |
|---|---|---|
| Leu 65 | Val 65 | conflict |
| Gln 146 | Glu 146 | engineered mutation |
| Ser 312 | Asn 312 | conflict |
| Thr 355 | Ala 355 | conflict |
| Val 407 | Leu 407 | conflict |
| Ile 440 | Leu 440 | conflict |
| Ala 534 | Thr 534 | conflict |
| Ser 583 | Pro 583 | conflict |
| Glu 598 | Gln 598 | engineered mutation |
| Arg 607 | His 607 | conflict |
| Leu 618 | Val 618 | conflict |
| Ser 619 | Phe 619 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 68879.1 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1761.3 | |
| All* | Total formula weight | 70640.3 |
