5N7J

Crystal structure of Neisseria polysaccharea amylosucrase mutant efficient for the synthesis of controlled size maltooligosaccharides

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Amylosucrase	polymer	628	71494.0	1	UniProt (Q9ZEU2) Pfam (PF00128) Pfam (PF22582) In PDB	Neisseria polysaccharea
2	B, C, D	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	3	In PDB BIRD (PRD_900003)		sucrose
3	E	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	1	In PDB BIRD (PRD_900009) GlyTouCan (G96370VA)		alpha-maltotriose
4	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5	A	PENTAETHYLENE GLYCOL	non-polymer		238.3	2	Chemie (1PE)
6	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
7		water	water		18.0	941	Chemie (HOH)

Sequence modifications

A: 5 - 628 (UniProt: Q9ZEU2)

PDB	External Database	Details
Ser 1	-	expression tag
Pro 2	-	expression tag
Asn 3	-	expression tag
Ser 4	-	expression tag
Lys 226	Arg 234	engineered mutation
Val 228	Ile 236	engineered mutation
Ile 289	Ala 297	engineered mutation
Tyr 290	Phe 298	engineered mutation
Ile 300	Glu 308	engineered mutation
Thr 331	Val 339	engineered mutation
Ser 437	Gln 445	engineered mutation
Asp 439	Asn 447	engineered mutation
Ala 445	Cys 453	engineered mutation
Asp 537	Gly 545	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	71494.0
Branched	Number of molecules	4
	Total formula weight	1531.3
Non-Polymers*	Number of molecules	4
	Total formula weight	792.9
All*	Total formula weight	73818.3

*Water molecules are not included.