5N6V
Crystal structure of Neisseria polysaccharea amylosucrase mutant derived from Neutral genetic Drift-based engineering
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Amylosucrase | polymer | 628 | 71616.1 | 1 | UniProt (Q9ZEU2) | Neisseria polysaccharea | |
| 2 | B, C (B, C) | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900003) | sucrose | ||
| 3 | D (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 4 | E (A) | alpha-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (GLC) | |||
| 5 | F (A) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 1 | Chemie (EPE) | |||
| 6 | G (A) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 7 | H, I (A) | beta-D-fructofuranose | non-polymer | 180.2 | 2 | Chemie (FRU) | |||
| 8 | J (A) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 9 | K (A) | water | water | 18.0 | 1169 | Chemie (HOH) |
Sequence modifications
A: 5 - 628 (UniProt: Q9ZEU2)
| PDB | External Database | Details |
|---|---|---|
| Ser 1 | - | expression tag |
| Pro 2 | - | expression tag |
| Asn 3 | - | expression tag |
| Ser 4 | - | expression tag |
| His 37 | Asp 45 | engineered mutation |
| Asp 331 | Val 339 | engineered mutation |
| Asn 526 | Asp 534 | engineered mutation |
| Asp 537 | Gly 545 | conflict |
| Ile 609 | Val 617 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 71616.1 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1289.4 | |
| All* | Total formula weight | 73590.1 |
