5N0F

The catalytic domain, BcGH76, of Bacillus circulans Aman6 in complex with 1,6-ManSIFG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Alpha-1,6-mannanase	polymer	362	40930.9	2	UniProt (Q9Z4P9) Pfam (PF03663)	Bacillus circulans
2	C, D (A, B)	[(3S,4R,5R)-4,5-dihydroxypiperidin-3-yl]methyl 1-thio-alpha-D-mannopyranoside	non-polymer		325.4	2	Chemie (7K2)
3	E, F (A, B)	water	water		18.0	559	Chemie (HOH)

Sequence modifications

A, B: 35 - 375 (UniProt: Q9Z4P9)

PDB	External Database	Details
Met 14	-	initiating methionine
Gly 15	-	expression tag
Ser 16	-	expression tag
Ser 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
Ser 24	-	expression tag
Ser 25	-	expression tag
Gly 26	-	expression tag
Leu 27	-	expression tag
Glu 28	-	expression tag
Val 29	-	expression tag
Leu 30	-	expression tag
Phe 31	-	expression tag
Gln 32	-	expression tag
Gly 33	-	expression tag
Pro 34	-	expression tag
Gln 341	Arg 341	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81861.7
Non-Polymers*	Number of molecules	2
	Total formula weight	650.8
All*	Total formula weight	82512.5

*Water molecules are not included.