5MZR
X-ray structure of the H235Q mutant of GLIC in complex with propofol
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E (A, B, C, D, E) | Proton-gated ion channel | polymer | 327 | 37408.9 | 5 | UniProt (Q7NDN8) Pfam (PF02931) | Gloeobacter violaceus (strain PCC 7421) | GLIC,Ligand-gated ion channel,LGIC |
| 2 | AA, BA, CA, F, G... (C, A, D, B, E) | DIUNDECYL PHOSPHATIDYL CHOLINE | non-polymer | 622.8 | 15 | Chemie (PLC) | |||
| 3 | DA, I, J, K, MA... (C, A, D, B, E) | CHLORIDE ION | non-polymer | 35.5 | 7 | Chemie (CL) | |||
| 4 | EA, L, NA, R, W... (C, A, D, B, E) | SODIUM ION | non-polymer | 23.0 | 6 | Chemie (NA) | |||
| 5 | FA, IA, M, OA, P... (C, D, A, E, B) | ACETATE ION | non-polymer | 59.0 | 10 | Chemie (ACT) | |||
| 6 | GA, N, O, PA, Y... (C, A, D, B, E) | DODECYL-BETA-D-MALTOSIDE | non-polymer | 510.6 | 6 | Chemie (LMT) | |||
| 7 | AB, HA, Q, RA (E, C, A, D) | 2,6-BIS(1-METHYLETHYL)PHENOL | non-polymer | 178.3 | 4 | Chemie (PFL) | |||
| 8 | BB, CB, DB, EB, FB (A, B, C, D, E) | water | water | 18.0 | 161 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E: 1 - 317 (UniProt: Q7NDN8)
| PDB | External Database | Details |
|---|---|---|
| Gln 235 | His 277 | engineered mutation |
| Gly 318 | - | expression tag |
| Tyr 319 | - | expression tag |
| Pro 320 | - | expression tag |
| Tyr 321 | - | expression tag |
| Asp 322 | - | expression tag |
| Val 323 | - | expression tag |
| Pro 324 | - | expression tag |
| Asp 325 | - | expression tag |
| Tyr 326 | - | expression tag |
| Ala 327 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 187044.4 | |
| Non-Polymers* | Number of molecules | 48 |
| Total formula weight | 14095.8 | |
| All* | Total formula weight | 201140.2 |
