5MY5

Tungstate binding protein - TupA - from Desulfovibrio alaskensis G20

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	ABC transporter periplasmic substrate-binding protein	polymer	274	29697.9	1	UniProt (Q316W1) Pfam (PF12849) In PDB	Desulfovibrio alaskensis (strain G20)
2	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
3	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4		water	water		18.0	332	Chemie (HOH)

Sequence modifications

A: 1 - 251 (UniProt: Q316W1)

PDB	External Database	Details
Met -22	-	initiating methionine
Ala -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Val -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Met -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag
Lys 107	Arg 127	engineered mutation
Pro 138	Ser 158	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	29697.9
Non-Polymers*	Number of molecules	3
	Total formula weight	93.9
All*	Total formula weight	29791.8

*Water molecules are not included.