5MS0

pseudo-atomic model of the RNA polymerase lambda-based antitermination complex solved by cryo-EM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (N)	Antitermination protein N	polymer	85	9877.3	1	UniProt (P03045) Pfam (PF11438)	Escherichia phage lambda (Bacteriophage lambda)	Regulatory protein N,PN
2	B (R)	nascent RNA	polymer	29	9339.6	1		Escherichia coli
3	C, D (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
4	E (C)	DNA-directed RNA polymerase subunit beta	polymer	1342	150804.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562)	Escherichia coli K-12	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
5	F (D)	DNA-directed RNA polymerase subunit beta'	polymer	1416	156537.0	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Escherichia coli K-12	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
6	G (E)	30S ribosomal protein S10	polymer	100	11340.1	1	UniProt (P0A7R5) Pfam (PF00338)	Escherichia coli K-12	Small ribosomal subunit protein uS10
7	H (F)	Transcription termination/antitermination protein NusG	polymer	183	20688.7	1	UniProt (P0AFG0) Pfam (PF02357) Pfam (PF00467)	Escherichia coli K-12
8	I (H)	RNA transcription bubble	polymer	14	4492.8	1		Escherichia coli
9	J (I)	DNAI	polymer	27	8355.4	1		Escherichia coli
10	K (J)	DNAII	polymer	39	11942.7	1		Escherichia coli
11	L (L)	Transcription antitermination protein NusB	polymer	139	15710.0	1	UniProt (P0A780) Pfam (PF01029)	Escherichia coli K-12	Antitermination factor NusB,N utilization substance protein B
12	M (M)	Transcription termination/antitermination protein NusA	polymer	497	55059.8	1	UniProt (P0AFF6) Pfam (PF08529) Pfam (PF00575) Pfam (PF13184) Pfam (PF14520)	Escherichia coli K-12	N utilization substance protein A,Transcription termination/antitermination L factor
13	N (O)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192)	Escherichia coli K-12	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
14	O (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
15	P, Q (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

N: 3 - 86 (UniProt: P03045)

PDB	External Database	Details
Ala 2	-	expression tag
Ala 56	Asp 56	conflict
Asp 57	Leu 57	conflict
Leu 58	Thr 58	conflict
Thr 59	Val 59	conflict
Val 60	Leu 60	conflict

C: 1 - 1342 (UniProt: P0A8V2)

PDB	External Database	Details
Val 516	Asp 516	conflict

D: 1 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Val 1410	-	expression tag
His 1411	-	expression tag
His 1412	-	expression tag
His 1413	-	expression tag
His 1414	-	expression tag
His 1415	-	expression tag
His 1416	-	expression tag

E: 4 - 103 (UniProt: P0A7R5)

PDB	External Database	Details
Ser 46	Lys 46	conflict

F: 1 - 181 (UniProt: P0AFG0)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

L: 1 - 139 (UniProt: P0A780)

PDB	External Database	Details
Glu 2	Lys 2	conflict

M: 1 - 555 (UniProt: P0AFF6)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Gln 462	Glu 422	conflict

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	537515.3
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	537670.4

*Water molecules are not included.