5MS0
pseudo-atomic model of the RNA polymerase lambda-based antitermination complex solved by cryo-EM
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | N | Antitermination protein N | polymer | 85 | 9877.3 | 1 | UniProt (P03045) Pfam (PF11438) In PDB | Escherichia phage lambda (Bacteriophage lambda) | Regulatory protein N,PN |
2 | R | nascent RNA | polymer | 29 | 9339.6 | 1 | Escherichia coli | ||
3 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
4 | C | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150804.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
5 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1416 | 156537.0 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
6 | E | 30S ribosomal protein S10 | polymer | 100 | 11340.1 | 1 | UniProt (P0A7R5) Pfam (PF00338) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS10 |
7 | F | Transcription termination/antitermination protein NusG | polymer | 183 | 20688.7 | 1 | UniProt (P0AFG0) Pfam (PF02357) Pfam (PF00467) In PDB | Escherichia coli K-12 | |
8 | H | RNA transcription bubble | polymer | 14 | 4492.8 | 1 | Escherichia coli | ||
9 | I | DNAI | polymer | 27 | 8355.4 | 1 | Escherichia coli | ||
10 | J | DNAII | polymer | 39 | 11942.7 | 1 | Escherichia coli | ||
11 | L | Transcription antitermination protein NusB | polymer | 139 | 15710.0 | 1 | UniProt (P0A780) Pfam (PF01029) In PDB | Escherichia coli K-12 | Antitermination factor NusB,N utilization substance protein B |
12 | M | Transcription termination/antitermination protein NusA | polymer | 497 | 55059.8 | 1 | UniProt (P0AFF6) Pfam (PF08529) Pfam (PF00575) Pfam (PF13184) Pfam (PF14520) In PDB | Escherichia coli K-12 | N utilization substance protein A,Transcription termination/antitermination L factor |
13 | O | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
14 | D | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
15 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
N: 3 - 86 (UniProt: P03045)
C: 1 - 1342 (UniProt: P0A8V2)
D: 1 - 1407 (UniProt: P0A8T7)
E: 4 - 103 (UniProt: P0A7R5)
F: 1 - 181 (UniProt: P0AFG0)
L: 1 - 139 (UniProt: P0A780)
M: 1 - 555 (UniProt: P0AFF6)
PDB | External Database | Details |
---|---|---|
Ala 2 | - | expression tag |
Ala 56 | Asp 56 | conflict |
Asp 57 | Leu 57 | conflict |
Leu 58 | Thr 58 | conflict |
Thr 59 | Val 59 | conflict |
Val 60 | Leu 60 | conflict |
PDB | External Database | Details |
---|---|---|
Val 516 | Asp 516 | conflict |
PDB | External Database | Details |
---|---|---|
Leu 1408 | - | expression tag |
Glu 1409 | - | expression tag |
Val 1410 | - | expression tag |
His 1411 | - | expression tag |
His 1412 | - | expression tag |
His 1413 | - | expression tag |
His 1414 | - | expression tag |
His 1415 | - | expression tag |
His 1416 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ser 46 | Lys 46 | conflict |
PDB | External Database | Details |
---|---|---|
Gly -1 | - | expression tag |
Ala 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Glu 2 | Lys 2 | conflict |
PDB | External Database | Details |
---|---|---|
Gly -1 | - | expression tag |
Ala 0 | - | expression tag |
Gln 462 | Glu 422 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 14 |
Total formula weight | 537515.3 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 155.1 | |
All* | Total formula weight | 537670.4 |