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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

5MQ0

Structure of a spliceosome remodeled for exon ligation

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(I)		Yeast UBC4 gene for ubiquitin-conjugating enzymepolymer9530200.71GenBank (4718)Saccharomyces cerevisiae (Baker's yeast)
2B
(E)		5'-EXON OF UBC4 PRE-MRNApolymer206519.01Saccharomyces cerevisiae (Baker's yeast)
3C
(2)		S.cerevisiae chromosome II reading frame ORF YBR230cpolymer1175376267.41GenBank (536627)Saccharomyces cerevisiae (Baker's yeast)
4D
(6)		Saccharomyces cerevisiae strain T.52_2H chromosome XII sequencepolymer11235883.21GenBank (1039022925)Saccharomyces cerevisiae (Baker's yeast)
5E
(5)		Saccharomyces cerevisiae strain WI_C_MBSP_4 chromosome VII sequencepolymer17957444.91GenBank (1039023924)Saccharomyces cerevisiae (Baker's yeast)
6F
(A)		Pre-mRNA-splicing factor 8polymer2413279867.51UniProt (P33334)
Pfam (PF08082)
Pfam (PF08083)
Pfam (PF10598)
Pfam (PF10597)
Pfam (PF10596)
Pfam (PF12134)
Pfam (PF08084)		Saccharomyces cerevisiae (Baker's yeast)
7G
(C)		Pre-mRNA-splicing factor SNU114polymer1008114174.01UniProt (P36048)
Pfam (PF03764)
Pfam (PF00679)
Pfam (PF16004)
Pfam (PF00009)		Saccharomyces cerevisiae (Baker's yeast)114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10
8H
(H)		Pre-mRNA-splicing factor CWC22polymer57767386.11UniProt (P53333)
Pfam (PF02847)		Saccharomyces cerevisiae (Baker's yeast)Complexed with CEF1 protein 22
9I
(J)		Pre-mRNA-splicing factor PRP46polymer45150771.31UniProt (Q12417)
Pfam (PF00400)		Saccharomyces cerevisiae (Baker's yeast)Complexed with CEF1 protein 1,PRP nineteen-associated complex protein 50,PRP19-associated complex protein 50,Pre-mRNA-processing protein 46
10J
(K)		Pre-mRNA-processing protein 45polymer37942548.71UniProt (P28004)
Pfam (PF02731)		Saccharomyces cerevisiae (Baker's yeast)
11K
(L)		Pre-mRNA-splicing factor BUD31polymer15718484.51UniProt (P25337)
Pfam (PF01125)		Saccharomyces cerevisiae (Baker's yeast)Bud site selection protein 31,Complexed with CEF1 protein 14
12L
(M)		Pre-mRNA-splicing factor CWC2polymer33938486.61UniProt (Q12046)
Pfam (PF16131)
Pfam (PF00076)		Saccharomyces cerevisiae (Baker's yeast)Complexed with CEF1 protein 2,PRP19-associated complex protein 40,Synthetic lethal with CLF1 protein 3
13M
(N)		Pre-mRNA-splicing factor SLT11polymer36440988.61UniProt (P38241)
Pfam (PF21369)		Saccharomyces cerevisiae (Baker's yeast)Extracellular mutant protein 2,Synthetic lethality with U2 protein 11
14N
(O)		Pre-mRNA-splicing factor CEF1polymer59067837.81UniProt (Q03654)
Pfam (PF00249)
Pfam (PF11831)		Saccharomyces cerevisiae (Baker's yeast)PRP nineteen-associated complex protein 85,PRP19-associated complex protein 85
15O
(P)		Pre-mRNA-splicing factor CWC15polymer17519975.21UniProt (Q03772)
Pfam (PF04889)		Saccharomyces cerevisiae (Baker's yeast)Complexed with CEF1 protein 15
16P
(R)		Pre-mRNA-splicing factor CWC21polymer13515793.61UniProt (Q03375)
Pfam (PF08312)		Saccharomyces cerevisiae (Baker's yeast)Complexed with CEF1 protein 21
17Q
(S)		Pre-mRNA-splicing factor CLF1polymer68782555.91UniProt (Q12309)
Pfam (PF23233)
Pfam (PF23240)		Saccharomyces cerevisiae (Baker's yeast)Crooked neck-like factor 1,PRP19-associated complex protein 77,Synthetic lethal with CDC40 protein 3
18R
(T)		Pre-mRNA-splicing factor SYF1,PRE-MRNA-SPLICING FACTOR SYF1polymer877101875.91UniProt (Q04048)
Pfam (PF23233)
Pfam (PF23220)
Pfam (PF23231)		Saccharomyces cerevisiae (Baker's yeast)PRP19-associated complex protein 90,Synthetic lethal with CDC40 protein 1,PRP19-associated complex protein 90,Synthetic lethal with CDC40 protein 1
19S
(a)		Pre-mRNA-splicing factor 18polymer25128414.41UniProt (P33411)
Pfam (PF02840)		Saccharomyces cerevisiae (Baker's yeast)
20T
(c)		Pre-mRNA-splicing factor SLU7polymer38244722.91UniProt (Q02775)
Pfam (PF11708)		Saccharomyces cerevisiae (Baker's yeast)Synthetic lethal with U2 snRNA protein 17,Synthetic lethal with U5 snRNA protein 7
21U
(o)		Pre-mRNA-processing factor 17polymer45552128.81UniProt (P40968)
Pfam (PF00400)		Saccharomyces cerevisiae (Baker's yeast)Cell division control protein 40
22V
(X)		UNKNOWN PROTEINpolymer685805.11Saccharomyces cerevisiae (Baker's yeast)
23W
(y)		Pre-mRNA-splicing factor SYF2polymer21524850.71UniProt (P53277)
Pfam (PF08231)		Saccharomyces cerevisiae (Baker's yeast)PRP19 complex protein 31,Synthetic lethal with CDC40 protein 2
24FA, X
(b, k)		Small nuclear ribonucleoprotein-associated protein Bpolymer19622427.02UniProt (P40018)
Pfam (PF01423)		Saccharomyces cerevisiae (Baker's yeast)snRNP-B,Sm protein B,SmB
25IA, Y
(d, n)		Small nuclear ribonucleoprotein Sm D3polymer10111240.12UniProt (P43321)
Pfam (PF01423)		Saccharomyces cerevisiae (Baker's yeast)Sm-D3,snRNP core protein D3
26MA, Z
(e, p)		Small nuclear ribonucleoprotein Epolymer9410385.12UniProt (Q12330)
Pfam (PF01423)		Saccharomyces cerevisiae (Baker's yeast)snRNP-E,Sm protein E,SmE
27AA, NA
(f, q)		Small nuclear ribonucleoprotein Fpolymer869669.92UniProt (P54999)
Pfam (PF01423)		Saccharomyces cerevisiae (Baker's yeast)snRNP-F,Sm protein F,SmF
28BA, JA
(g, r)		Small nuclear ribonucleoprotein Gpolymer778490.82UniProt (P40204)
Pfam (PF01423)		Saccharomyces cerevisiae (Baker's yeast)snRNP-G,Sm protein G,SmG
29CA, GA
(h, l)		Small nuclear ribonucleoprotein Sm D1polymer14616296.82UniProt (Q02260)
Pfam (PF01423)		Saccharomyces cerevisiae (Baker's yeast)Sm-D1,snRNP core protein D1
30DA, HA
(j, m)		Small nuclear ribonucleoprotein Sm D2polymer11012876.12UniProt (Q06217)
Pfam (PF01423)		Saccharomyces cerevisiae (Baker's yeast)Sm-D2,snRNP core protein D2
31EA
(V)		Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22polymer1145130187.41UniProt (P24384)
Pfam (PF00575)
Pfam (PF00270)
Pfam (PF00271)
Pfam (PF04408)
Pfam (PF21010)
Pfam (PF07717)		Saccharomyces cerevisiae (Baker's yeast)
32KA
(W)		U2 small nuclear ribonucleoprotein A'polymer23827232.31UniProt (Q08963)
Pfam (PF14580)		Saccharomyces cerevisiae (Baker's yeast)U2 snRNP A',Looks exceptionally like U2A protein 1
33LA
(Y)		U2 small nuclear ribonucleoprotein B''polymer11112850.91UniProt (P40567)
Pfam (PF13893)		Saccharomyces cerevisiae (Baker's yeast)U2 snRNP B''
34OA
(3)		3'-EXON OF UBC4 PRE-MRNA, BOUND BY PRP22 HELICASEpolymer3873.51Saccharomyces cerevisiae (Baker's yeast)
35PA
(s)		Pre-mRNA-splicing factor SNT309polymer17520741.51UniProt (Q06091)Saccharomyces cerevisiae (Baker's yeast)PRP19-associated complex protein 25,Synergistic to PRP19 mutation protein 309
36QA, RA, SA, TA
(t, u, v, w)		Pre-mRNA-processing factor 19polymer50356629.84UniProt (P32523)
Pfam (PF08606)		Saccharomyces cerevisiae (Baker's yeast)
37UA, VA, WA
(6)		MAGNESIUM IONnon-polymer24.33Chemie (MG)
PubChem (888)
38XA, YA
(6)		POTASSIUM IONnon-polymer39.12Chemie (K)
PubChem (813)
39ZA
(A)		INOSITOL HEXAKISPHOSPHATEnon-polymer660.01Chemie (IHP)
40AB
(C)		GUANOSINE-5'-TRIPHOSPHATEnon-polymer523.21Chemie (GTP)
PubChem (177679032)
PubChem (135400627)
PubChem (135487750)
PubChem (136088935)
PubChem (136803486)
PubChem (135398633)
PubChem (135544356)
PubChem (135547949)
PubChem (135600366)
PubChem (135749822)
PubChem (136005188)
PubChem (136073923)
PubChem (136240914)
PubChem (136664747)
PubChem (136772743)
PubChem (136772744)
PubChem (136803487)
PubChem (137286749)
PubChem (164715984)
PubChem (171699466)
41BB, CB, DB, EB, FB...
(L, M, N)		ZINC IONnon-polymer65.46Chemie (ZN)
Sequence modifications
T: 1 - 859 (UniProt: Q04048)
T: 1000 - 1017 (PDB: 5MQ0)
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains46
Total formula weight2204158.9
Non-Polymers*Number of molecules13
Total formula weight1726.8
All*Total formula weight2205885.7
*Water molecules are not included.

253389

PDB entries from 2026-05-13

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