5MQ0
Structure of a spliceosome remodeled for exon ligation
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | I | Yeast UBC4 gene for ubiquitin-conjugating enzyme | polymer | 95 | 30200.7 | 1 | GenBank (4718) | Saccharomyces cerevisiae (Baker's yeast) | |
2 | E | 5'-EXON OF UBC4 PRE-MRNA | polymer | 20 | 6519.0 | 1 | Saccharomyces cerevisiae (Baker's yeast) | ||
3 | 2 | S.cerevisiae chromosome II reading frame ORF YBR230c | polymer | 1175 | 376267.4 | 1 | GenBank (536627) | Saccharomyces cerevisiae (Baker's yeast) | |
4 | 6 | Saccharomyces cerevisiae strain T.52_2H chromosome XII sequence | polymer | 112 | 35883.2 | 1 | GenBank (1039022925) | Saccharomyces cerevisiae (Baker's yeast) | |
5 | 5 | Saccharomyces cerevisiae strain WI_C_MBSP_4 chromosome VII sequence | polymer | 179 | 57444.9 | 1 | GenBank (1039023924) | Saccharomyces cerevisiae (Baker's yeast) | |
6 | A | Pre-mRNA-splicing factor 8 | polymer | 2413 | 279867.5 | 1 | UniProt (P33334) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) In PDB | Saccharomyces cerevisiae (Baker's yeast) | |
7 | C | Pre-mRNA-splicing factor SNU114 | polymer | 1008 | 114174.0 | 1 | UniProt (P36048) Pfam (PF03764) Pfam (PF00679) Pfam (PF16004) Pfam (PF00009) In PDB | Saccharomyces cerevisiae (Baker's yeast) | 114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10 |
8 | H | Pre-mRNA-splicing factor CWC22 | polymer | 577 | 67386.1 | 1 | UniProt (P53333) Pfam (PF02847) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Complexed with CEF1 protein 22 |
9 | J | Pre-mRNA-splicing factor PRP46 | polymer | 451 | 50771.3 | 1 | UniProt (Q12417) Pfam (PF00400) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Complexed with CEF1 protein 1,PRP nineteen-associated complex protein 50,PRP19-associated complex protein 50,Pre-mRNA-processing protein 46 |
10 | K | Pre-mRNA-processing protein 45 | polymer | 379 | 42548.7 | 1 | UniProt (P28004) Pfam (PF02731) In PDB | Saccharomyces cerevisiae (Baker's yeast) | |
11 | L | Pre-mRNA-splicing factor BUD31 | polymer | 157 | 18484.5 | 1 | UniProt (P25337) Pfam (PF01125) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Bud site selection protein 31,Complexed with CEF1 protein 14 |
12 | M | Pre-mRNA-splicing factor CWC2 | polymer | 339 | 38486.6 | 1 | UniProt (Q12046) Pfam (PF16131) Pfam (PF00076) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Complexed with CEF1 protein 2,PRP19-associated complex protein 40,Synthetic lethal with CLF1 protein 3 |
13 | N | Pre-mRNA-splicing factor SLT11 | polymer | 364 | 40988.6 | 1 | UniProt (P38241) Pfam (PF21369) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Extracellular mutant protein 2,Synthetic lethality with U2 protein 11 |
14 | O | Pre-mRNA-splicing factor CEF1 | polymer | 590 | 67837.8 | 1 | UniProt (Q03654) Pfam (PF00249) Pfam (PF11831) In PDB | Saccharomyces cerevisiae (Baker's yeast) | PRP nineteen-associated complex protein 85,PRP19-associated complex protein 85 |
15 | P | Pre-mRNA-splicing factor CWC15 | polymer | 175 | 19975.2 | 1 | UniProt (Q03772) Pfam (PF04889) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Complexed with CEF1 protein 15 |
16 | R | Pre-mRNA-splicing factor CWC21 | polymer | 135 | 15793.6 | 1 | UniProt (Q03375) Pfam (PF08312) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Complexed with CEF1 protein 21 |
17 | S | Pre-mRNA-splicing factor CLF1 | polymer | 687 | 82555.9 | 1 | UniProt (Q12309) Pfam (PF05843) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Crooked neck-like factor 1,PRP19-associated complex protein 77,Synthetic lethal with CDC40 protein 3 |
18 | T | Pre-mRNA-splicing factor SYF1,PRE-MRNA-SPLICING FACTOR SYF1 | polymer | 877 | 101875.9 | 1 | UniProt (Q04048) Pfam (PF05843) In PDB | Saccharomyces cerevisiae (Baker's yeast) | PRP19-associated complex protein 90,Synthetic lethal with CDC40 protein 1,PRP19-associated complex protein 90,Synthetic lethal with CDC40 protein 1 |
19 | a | Pre-mRNA-splicing factor 18 | polymer | 251 | 28414.4 | 1 | UniProt (P33411) Pfam (PF02840) In PDB | Saccharomyces cerevisiae (Baker's yeast) | |
20 | c | Pre-mRNA-splicing factor SLU7 | polymer | 382 | 44722.9 | 1 | UniProt (Q02775) Pfam (PF11708) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Synthetic lethal with U2 snRNA protein 17,Synthetic lethal with U5 snRNA protein 7 |
21 | o | Pre-mRNA-processing factor 17 | polymer | 455 | 52128.8 | 1 | UniProt (P40968) Pfam (PF00400) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Cell division control protein 40 |
22 | X | UNKNOWN PROTEIN | polymer | 68 | 5805.1 | 1 | Saccharomyces cerevisiae (Baker's yeast) | ||
23 | y | Pre-mRNA-splicing factor SYF2 | polymer | 215 | 24850.7 | 1 | UniProt (P53277) Pfam (PF08231) In PDB | Saccharomyces cerevisiae (Baker's yeast) | PRP19 complex protein 31,Synthetic lethal with CDC40 protein 2 |
24 | b, k | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 2 | UniProt (P40018) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | snRNP-B,Sm protein B,SmB |
25 | d, n | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 2 | UniProt (P43321) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Sm-D3,snRNP core protein D3 |
26 | e, p | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 2 | UniProt (Q12330) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | snRNP-E,Sm protein E,SmE |
27 | f, q | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 2 | UniProt (P54999) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | snRNP-F,Sm protein F,SmF |
28 | g, r | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 2 | UniProt (P40204) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | snRNP-G,Sm protein G,SmG |
29 | h, l | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 2 | UniProt (Q02260) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Sm-D1,snRNP core protein D1 |
30 | j, m | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 2 | UniProt (Q06217) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Sm-D2,snRNP core protein D2 |
31 | V | Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 | polymer | 1145 | 130187.4 | 1 | UniProt (P24384) Pfam (PF00575) Pfam (PF00270) Pfam (PF00271) Pfam (PF04408) Pfam (PF21010) Pfam (PF07717) In PDB | Saccharomyces cerevisiae (Baker's yeast) | |
32 | W | U2 small nuclear ribonucleoprotein A' | polymer | 238 | 27232.3 | 1 | UniProt (Q08963) Pfam (PF14580) In PDB | Saccharomyces cerevisiae (Baker's yeast) | U2 snRNP A',Looks exceptionally like U2A protein 1 |
33 | Y | U2 small nuclear ribonucleoprotein B'' | polymer | 111 | 12850.9 | 1 | UniProt (P40567) Pfam (PF13893) In PDB | Saccharomyces cerevisiae (Baker's yeast) | U2 snRNP B'' |
34 | 3 | 3'-EXON OF UBC4 PRE-MRNA, BOUND BY PRP22 HELICASE | polymer | 3 | 873.5 | 1 | Saccharomyces cerevisiae (Baker's yeast) | ||
35 | s | Pre-mRNA-splicing factor SNT309 | polymer | 175 | 20741.5 | 1 | UniProt (Q06091) In PDB | Saccharomyces cerevisiae (Baker's yeast) | PRP19-associated complex protein 25,Synergistic to PRP19 mutation protein 309 |
36 | t, u, v, w | Pre-mRNA-processing factor 19 | polymer | 503 | 56629.8 | 4 | UniProt (P32523) Pfam (PF08606) In PDB | Saccharomyces cerevisiae (Baker's yeast) | |
37 | 6 | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
38 | 6 | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
39 | A | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 1 | Chemie (IHP) | |||
40 | C | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
41 | L, M, N | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) |
Sequence modifications
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 46 |
Total formula weight | 2204158.9 | |
Non-Polymers* | Number of molecules | 13 |
Total formula weight | 1726.8 | |
All* | Total formula weight | 2205885.7 |