5MLY

Closed loop conformation of PhaZ7 Y105E mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	PHB depolymerase PhaZ7	polymer	350	37274.1	2	UniProt (Q939Q9) Pfam (PF01674) In PDB	Paucimonas lemoignei
2		water	water		18.0	1202	Chemie (HOH)

Sequence modifications

A, B: 1 - 342 (UniProt: Q939Q9)

PDB	External Database	Details
Glu 105	Tyr 143	engineered mutation
Leu 343	-	expression tag
Glu 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74548.2
All*	Total formula weight	74548.2

*Water molecules are not included.