5MJE

Crystal structure of the HigB2 toxin in complex with Nb8

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cytotoxic translational repressor of toxin-antitoxin stability system	polymer	129	15066.1	1	UniProt (A0A085RXT0) Pfam (PF06296) UniProt (by SIFTS) (Q9KMA6)	Vibrio cholerae	Toxin HigB-2,Uncharacterized protein
2	B (B)	Nanobody 8	polymer	131	14367.8	1		Lama glama
3	C (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4	D (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	E, F (A, B)	water	water		18.0	17	Chemie (HOH)

Sequence modifications

A: 1 - 110 (UniProt: A0A085RXT0)

PDB	External Database	Details
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	29433.9
Non-Polymers*	Number of molecules	2
	Total formula weight	202.2
All*	Total formula weight	29636.1

*Water molecules are not included.