5MGP

Structural basis for ArfA-RF2 mediated translation termination on stop-codon lacking mRNAs

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	23S ribosomal RNA	polymer	2903	941305.3	1	GenBank (802133627)	Escherichia coli
2	B (B)	5S ribosomal RNA	polymer	120	38813.1	1	GenBank (1028480521)	Escherichia coli
3	C (C)	50S ribosomal protein L2	polymer	271	29663.2	1	UniProt (P60422) Pfam (PF00181) Pfam (PF03947)	Escherichia coli
4	D (D)	50S ribosomal protein L3	polymer	209	22277.5	1	UniProt (P60438) Pfam (PF00297)	Escherichia coli
5	E (E)	50S ribosomal protein L4	polymer	201	22121.6	1	UniProt (P60723) Pfam (PF00573)	Escherichia coli
6	F (F)	50S ribosomal protein L5	polymer	177	20073.2	1	UniProt (P62399) Pfam (PF00281) Pfam (PF00673)	Escherichia coli
7	G (G)	50S ribosomal protein L6	polymer	176	18801.6	1	UniProt (P0AG55) Pfam (PF00347)	Escherichia coli
8	H (H)	50S ribosomal protein L9	polymer	149	15789.0	1	UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948)	Escherichia coli
9	I (J)	50S ribosomal protein L13	polymer	142	16050.6	1	UniProt (P0AA10) Pfam (PF00572)	Escherichia coli
10	J (K)	50S ribosomal protein L14	polymer	122	13451.9	1	UniProt (P0ADY3) Pfam (PF00238)	Escherichia coli
11	K (L)	50S ribosomal protein L15	polymer	143	14877.3	1	UniProt (P02413) Pfam (PF00828)	Escherichia coli
12	L (M)	50S ribosomal protein L16	polymer	136	15312.3	1	UniProt (P0ADY7) Pfam (PF00252)	Escherichia coli
13	M (N)	50S ribosomal protein L17	polymer	120	13721.9	1	UniProt (P0AG44) Pfam (PF01196)	Escherichia coli
14	N (O)	50S ribosomal protein L18	polymer	116	12663.5	1	UniProt (P0C018) Pfam (PF00861)	Escherichia coli
15	O (P)	50S ribosomal protein L19	polymer	114	13028.1	1	UniProt (P0A7K6) Pfam (PF01245)	Escherichia coli
16	P (Q)	50S ribosomal protein L20	polymer	117	13396.8	1	UniProt (P0A7L3) Pfam (PF00453)	Escherichia coli
17	Q (R)	50S ribosomal protein L21	polymer	103	11586.4	1	UniProt (P0AG48) Pfam (PF00829)	Escherichia coli
18	R (S)	50S ribosomal protein L22	polymer	110	12253.4	1	UniProt (P61175) Pfam (PF00237)	Escherichia coli
19	S (T)	50S ribosomal protein L23	polymer	93	10546.5	1	UniProt (P0ADZ0) Pfam (PF00276)	Escherichia coli
20	T (U)	50S ribosomal protein L24	polymer	102	11078.9	1	UniProt (P60624) Pfam (PF00467) Pfam (PF17136)	Escherichia coli
21	U (V)	50S ribosomal protein L25	polymer	94	10713.5	1	UniProt (P68919) Pfam (PF01386)	Escherichia coli
22	V (W)	50S ribosomal protein L27	polymer	75	8174.4	1	UniProt (P0A7L8) Pfam (PF01016)	Escherichia coli
23	W (X)	50S ribosomal protein L28	polymer	77	8896.4	1	UniProt (P0A7M2) Pfam (PF00830)	Escherichia coli
24	X (Y)	50S ribosomal protein L29	polymer	63	7286.5	1	UniProt (P0A7M6) Pfam (PF00831)	Escherichia coli
25	Y (Z)	50S ribosomal protein L30	polymer	58	6423.6	1	UniProt (P0AG51) Pfam (PF00327)	Escherichia coli
26	Z (0)	50S ribosomal protein L32	polymer	56	6332.2	1	UniProt (P0A7N4) Pfam (PF01783)	Escherichia coli
27	AA (1)	50S ribosomal protein L33	polymer	50	5814.8	1	UniProt (P0A7N9) Pfam (PF00471)	Escherichia coli
28	BA (2)	50S ribosomal protein L34	polymer	46	5397.5	1	UniProt (P0A7P5) Pfam (PF00468)	Escherichia coli
29	CA (3)	50S ribosomal protein L35	polymer	64	7181.8	1	UniProt (P0A7Q1) Pfam (PF01632)	Escherichia coli	Ribosomal protein A
30	DA (4)	50S ribosomal protein L36	polymer	38	4377.4	1	UniProt (P0A7Q6) Pfam (PF00444)	Escherichia coli	Ribosomal protein B
31	EA (6)	50S ribosomal protein L31	polymer	66	7516.7	1	UniProt (P0A7M9) Pfam (PF01197)	Escherichia coli
32	FA (a)	16S ribosomal RNA	polymer	1539	498725.4	1	GenBank (802133627)	Escherichia coli
33	GA (b)	30S ribosomal protein S2	polymer	218	24253.9	1	UniProt (P0A7V0) Pfam (PF00318)	Escherichia coli
34	HA (c)	30S ribosomal protein S3	polymer	206	23078.8	1	UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189)	Escherichia coli
35	IA (d)	30S ribosomal protein S4	polymer	205	23383.0	1	UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479)	Escherichia coli
36	JA (e)	30S ribosomal protein S5	polymer	157	16532.1	1	UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719)	Escherichia coli
37	KA (f)	30S ribosomal protein S6	polymer	100	11669.4	1	UniProt (P02358) Pfam (PF01250)	Escherichia coli
38	LA (g)	30S ribosomal protein S7	polymer	151	16861.5	1	UniProt (P02359) Pfam (PF00177)	Escherichia coli
39	MA (h)	30S ribosomal protein S8	polymer	129	14015.4	1	UniProt (P0A7W7) Pfam (PF00410)	Escherichia coli
40	NA (i)	30S ribosomal protein S9	polymer	127	14554.9	1	UniProt (P0A7X3) Pfam (PF00380)	Escherichia coli
41	OA (j)	30S ribosomal protein S10	polymer	98	11197.0	1	UniProt (P0A7R5) Pfam (PF00338)	Escherichia coli
42	PA (k)	30S ribosomal protein S11	polymer	116	12388.1	1	UniProt (P0A7R9) Pfam (PF00411)	Escherichia coli
43	QA (l)	30S ribosomal protein S12	polymer	123	13637.0	1	UniProt (P0A7S3) Pfam (PF00164)	Escherichia coli
44	RA (m)	30S ribosomal protein S13	polymer	114	12625.8	1	UniProt (P0A7S9) Pfam (PF00416)	Escherichia coli
45	SA (n)	30S ribosomal protein S14	polymer	101	11546.4	1	UniProt (P0AG59) Pfam (PF00253)	Escherichia coli
46	TA (o)	30S ribosomal protein S15	polymer	88	10159.6	1	UniProt (P0ADZ4) Pfam (PF00312)	Escherichia coli
47	UA (p)	30S ribosomal protein S16	polymer	82	9207.6	1	UniProt (P0A7T3) Pfam (PF00886)	Escherichia coli
48	VA (q)	30S ribosomal protein S17	polymer	80	9263.9	1	UniProt (P0AG63) Pfam (PF00366)	Escherichia coli
49	WA (r)	30S ribosomal protein S18	polymer	65	7606.8	1	UniProt (P0A7T7) Pfam (PF01084)	Escherichia coli
50	XA (s)	30S ribosomal protein S19	polymer	79	9057.6	1	UniProt (P0A7U3) Pfam (PF00203)	Escherichia coli
51	YA (t)	30S ribosomal protein S20	polymer	85	9506.2	1	UniProt (P0A7U7) Pfam (PF01649)	Escherichia coli
52	ZA (u)	30S ribosomal protein S21	polymer	65	7763.1	1	UniProt (P68679) Pfam (PF01165)	Escherichia coli
53	AB (v)	mRNA	polymer	6	1866.2	1		Escherichia coli
54	BB (w)	Alternative ribosome-rescue factor A	polymer	45	5265.1	1	UniProt (P36675) Pfam (PF03889)	Escherichia coli
55	CB (x)	P-site tRNA	polymer	77	24846.7	1	GenBank (1052521625)	Escherichia coli
56	DB (z)	Peptide chain release factor 2	polymer	359	40566.8	1	UniProt (P07012) Pfam (PF03462) Pfam (PF00472)	Escherichia coli	RF-2

Sequence modifications

A: 1 - 2903 (GenBank: 802133627)

PDB	External Database	Details
C 747	U 2724742	conflict
G 1847	A 2723642	conflict
A 2069	G 2723420	conflict

B: 1 - 120 (GenBank: 1028480521)

PDB	External Database	Details
A 120	U 834456	conflict

n: 1 - 101 (UniProt: P0AG59)

PDB	External Database	Details
Ala 35	-	insertion

z: 6 - 364 (UniProt: P07012)

PDB	External Database	Details
Ala 251	Gly 251	conflict

Contents of the asymmetric unit

Polymers	Number of chains	56
	Total formula weight	2184505.0
All*	Total formula weight	2184505.0

*Water molecules are not included.