5MEH

Crystal structure of alpha-1,2-mannosidase from Caulobacter K31 strain in complex with 1-deoxymannojirimycin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Mannosyl-oligosaccharide 1,2-alpha-mannosidase	polymer	447	50546.7	1	UniProt (B0SWV2) Pfam (PF01532)	Caulobacter sp.
2	B, C (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	D, E (A)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	F (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	G (A)	1-DEOXYMANNOJIRIMYCIN	non-polymer		163.2	1	Chemie (DMJ)
6	H (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
7	I (A)	water	water		18.0	505	Chemie (HOH)

Sequence modifications

A: 27 - 462 (UniProt: B0SWV2)

PDB	External Database	Details
Met 24	-	initiating methionine
Ala 25	-	expression tag
Ser 26	-	expression tag
Leu 463	-	expression tag
Glu 464	-	expression tag
His 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	50546.7
Non-Polymers*	Number of molecules	7
	Total formula weight	509.5
All*	Total formula weight	51056.2

*Water molecules are not included.