5MBV

Cryo-EM structure of Lambda Phage protein GamS bound to RecBCD.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	RecBCD enzyme subunit RecB	polymer	1181	134167.7	1	UniProt (P08394) Pfam (PF00580) Pfam (PF13361) Pfam (PF12705) In PDB	Escherichia coli	Exodeoxyribonuclease V 135 kDa polypeptide,Exodeoxyribonuclease V beta chain,Exonuclease V subunit RecB,ExoV subunit RecB,Helicase/nuclease RecBCD subunit RecB
2	C	RecBCD enzyme subunit RecC	polymer	1122	128974.1	1	UniProt (P07648) Pfam (PF04257) Pfam (PF17946) In PDB	Escherichia coli	Exodeoxyribonuclease V 125 kDa polypeptide,Exodeoxyribonuclease V gamma chain,Exonuclease V subunit RecC,ExoV subunit RecC
3	D	RecBCD enzyme subunit RecD	polymer	609	67047.4	1	UniProt (P04993) Pfam (PF21185) Pfam (PF13245) Pfam (PF13538) In PDB	Escherichia coli	Exodeoxyribonuclease V 67 kDa polypeptide,Exodeoxyribonuclease V alpha chain,Exonuclease V subunit RecD,ExoV subunit RecD,Helicase/nuclease RecBCD subunit RecD
4	A, E	Host-nuclease inhibitor protein gam	polymer	98	11661.9	2	UniProt (P03702) Pfam (PF06064) In PDB	Enterobacteria phage lambda

Sequence modifications

B: 1 - 1180 (UniProt: P08394)

PDB	External Database	Details
Gly 0	-	expression tag

D: 2 - 608 (UniProt: P04993)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 1	-	expression tag

A, E: 41 - 138 (UniProt: P03702)

PDB	External Database	Details
Ile 79	Leu 79	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	353513.1
All*	Total formula weight	353513.1

*Water molecules are not included.