5MAD
GFP-binding DARPin 3G61
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G (A, C, E, G) | 3G61 | polymer | 160 | 17017.0 | 4 | synthetic construct | ||
| 2 | B, D, F, H (B, D, F, H) | Green fluorescent protein | polymer | 245 | 27453.9 | 4 | UniProt (P42212) Pfam (PF01353) | Aequorea victoria (Jellyfish) | |
| 3 | I, J, K, L, M... (A, B, C, D, E...) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 8 | Chemie (PEG) | |||
| 4 | P (E) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 5 | R, S, T, U, V... (A, B, C, D, E...) | water | water | 18.0 | 1246 | Chemie (HOH) |
Sequence modifications
B, D, F, H: 2 - 238 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Met 0 | - | expression tag |
| Val 1 | - | expression tag |
| Leu 64 | Phe 64 | conflict |
| Cro 66 | Ser 65 | chromophore |
| Cro 66 | Tyr 66 | chromophore |
| Cro 66 | Gly 67 | chromophore |
| Leu 231 | His 231 | conflict |
| Gln 239 | - | expression tag |
| Ala 240 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 177883.9 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 872.0 | |
| All* | Total formula weight | 178755.9 |
