5M7Y

Crystal structure of GH125 1,6-alpha-mannosidase mutant from Clostridium perfringens in complex with 1,6-alpha-mannotriose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	1,6-alpha-mannosidase	polymer	435	50861.0	1	UniProt (Q8XNB2) Pfam (PF06824) In PDB	Clostridium perfringens (strain 13 / Type A)
2	B	alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose	branched		504.4	1	In PDB GlyTouCan (G85415LL)
3	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4		water	water		18.0	293	Chemie (HOH)

Sequence modifications

A: 1 - 427 (UniProt: Q8XNB2)

PDB	External Database	Details
Asn 220	Asp 220	engineered mutation
Leu 428	-	expression tag
Glu 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	50861.0
Branched	Number of molecules	1
	Total formula weight	504.4
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	51414.1

*Water molecules are not included.