5M6R

Human porphobilinogen deaminase in complex with reaction intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Porphobilinogen deaminase	polymer	389	42718.7	2	UniProt (P08397) Pfam (PF01379) Pfam (PF03900)	Homo sapiens (Human)	PBG-D,Hydroxymethylbilane synthase,HMBS,Pre-uroporphyrinogen synthase
2	C, G (A, B)	3-[4-(2-hydroxy-2-oxoethyl)-5-[[4-(2-hydroxy-2-oxoethyl)-5-[[4-(2-hydroxy-2-oxoethyl)-5-[[4-(2-hydroxy-2-oxoethyl)-3-(3-hydroxy-3-oxopropyl)-5-methyl-1~{H}-pyrrol-2-yl]methyl]-3-(3-hydroxy-3-oxopropyl)-1~{H}-pyrrol-2-yl]methyl]-3-(3-hydroxy-3-oxopropyl)-1~{H}-pyrrol-2-yl]methyl]-1~{H}-pyrrol-3-yl]propanoic acid	non-polymer		838.8	2	Chemie (7J8)
3	D, E, H (A, B)	PHOSPHATE ION	non-polymer		95.0	3	Chemie (PO4)
4	F (A)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
5	I, J (A, B)	water	water		18.0	23	Chemie (HOH)

Sequence modifications

A, B: 1 - 361 (UniProt: P08397)

PDB	External Database	Details
Met -27	-	initiating methionine
Lys -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
Pro -19	-	expression tag
Met -18	-	expression tag
Ser -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Asp -14	-	expression tag
Ile -13	-	expression tag
Pro -12	-	expression tag
Thr -11	-	expression tag
Thr -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	85437.4
Non-Polymers*	Number of molecules	6
	Total formula weight	2200.8
All*	Total formula weight	87638.3

*Water molecules are not included.