5M5I
Pseudo-atomic model of microtubule-bound S.pombe kinesin-5 motor domain in the AMPPNP state (based on cryo-electron microscopy experiment): the N-terminus conformation allows formation of a cover neck bundle.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Tubulin alpha-1D chain | polymer | 451 | 50107.2 | 1 | UniProt (Q2HJ86) Pfam (PF00091) Pfam (PF03953) | Bos taurus (Bovine) | |
| 2 | B (B) | Tubulin beta-2B chain | polymer | 445 | 49907.8 | 1 | UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) | Bos taurus (Bovine) | |
| 3 | C (C) | Kinesin-like protein cut7 | polymer | 369 | 40737.5 | 1 | UniProt (P24339) Pfam (PF00225) | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | Cell untimely torn protein 7 |
| 4 | D, H (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | E (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 6 | F (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 7 | G (B) | TAXOL | non-polymer | 853.9 | 1 | Chemie (TA1) | |||
| 8 | I (C) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) |
Sequence modifications
A: 1 - 451 (UniProt: Q2HJ86)
B: 1 - 445 (UniProt: Q6B856)
| PDB | External Database | Details |
|---|---|---|
| Ile 7 | Val 7 | conflict |
| Ile 114 | Leu 114 | conflict |
| Ser 136 | Leu 136 | conflict |
| Val 137 | Ile 137 | conflict |
| Gly 265 | Ile 265 | conflict |
| Glu 358 | Gln 358 | conflict |
| Val 437 | Met 437 | conflict |
| Gly 448 | Glu 448 | conflict |
| Glu 449 | Gly 449 | conflict |
| Glu 450 | Asp 450 | conflict |
| Tyr 451 | Glu 451 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 55 | Thr 55 | conflict |
| Val 170 | Met 170 | conflict |
| Ala 296 | Ser 296 | conflict |
| Val 316 | Ile 316 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 140752.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2375.1 | |
| All* | Total formula weight | 143127.6 |
