5M1S

Cryo-EM structure of the E. coli replicative DNA polymerase-clamp-exonuclase-theta complex bound to DNA in the editing mode

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase III subunit alpha	polymer	927	103554.4	1	UniProt (P10443) Pfam (PF02811) Pfam (PF07733) Pfam (PF17657) Pfam (PF14579) In PDB	Escherichia coli K12
2	B, C	DNA polymerase III subunit beta	polymer	366	40630.5	2	UniProt (P0A988) Pfam (PF00712) Pfam (PF02767) Pfam (PF02768) In PDB	Escherichia coli K12	Beta sliding clamp,Beta clamp
3	D	DNA polymerase III subunit epsilon	polymer	243	27119.0	1	UniProt (P03007) Pfam (PF00929) In PDB	Escherichia coli K12
4	P	DNA Primer Strand	polymer	17	5275.4	1		synthetic construct
5	T	DNA Template Strand	polymer	22	6718.3	1		synthetic construct
6	F	DNA polymerase III subunit theta	polymer	56	6503.4	1	UniProt (P0ABS8) Pfam (PF06440) In PDB	Escherichia coli K12

Sequence modifications

A: 1 - 927 (UniProt: P10443)

PDB	External Database	Details
Leu 921	Ala 921	engineered mutation
Leu 923	Met 923	engineered mutation

D: 1 - 243 (UniProt: P03007)

PDB	External Database	Details
Leu 183	Thr 183	engineered mutation
Leu 185	Met 185	engineered mutation
Pro 186	Ala 186	engineered mutation
Leu 187	Phe 187	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	230431.6
All*	Total formula weight	230431.6

*Water molecules are not included.