5M12

Structure of GH36 alpha-galactosidase from Thermotoga maritima in complex with intact cyclopropyl-carbasugar.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alpha-galactosidase	polymer	575	66198.1	1	UniProt (O33835) Pfam (PF02065) UniProt (by SIFTS) (G4FEF4) In PDB	Thermotoga maritima
2	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	A	(1~{R},2~{S},3~{S},4~{R},5~{S},6~{S})-5-[3,5-bis(fluoranyl)phenoxy]-1-(hydroxymethyl)bicyclo[4.1.0]heptane-2,3,4-triol	non-polymer		302.3	1	Chemie (7D0)
5		water	water		18.0	425	Chemie (HOH)

Sequence modifications

A: 1 - 552 (UniProt: O33835)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	66198.1
Non-Polymers*	Number of molecules	4
	Total formula weight	518.7
All*	Total formula weight	66716.8

*Water molecules are not included.