5M0O

Crystal structure of cytochrome P450 OleT H85Q in complex with arachidonic acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Terminal olefin-forming fatty acid decarboxylase	polymer	422	48418.9	1	UniProt (E9NSU2) Pfam (PF00067)	Jeotgalicoccus sp. ATCC 8456
2	B (C)	Terminal olefin-forming fatty acid decarboxylase	polymer	428	49247.8	1	UniProt (E9NSU2) Pfam (PF00067)	Jeotgalicoccus sp. ATCC 8456
3	C, F (A, C)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
4	D, G (A, C)	5,8,11,14,17-EICOSAPENTAENOIC ACID	non-polymer		302.5	2	Chemie (EPA)
5	E (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
6	H, I (A, C)	water	water		18.0	550	Chemie (HOH)

Sequence modifications

A: 1 - 422 (UniProt: E9NSU2)

PDB	External Database	Details
Gln 85	His 85	engineered mutation

C: 1 - 422 (UniProt: E9NSU2)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Gln 85	His 85	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97666.6
Non-Polymers*	Number of molecules	5
	Total formula weight	1933.9
All*	Total formula weight	99600.6

*Water molecules are not included.