5M03

Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-noeuromycin and 1,2-alpha-mannobiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glycosyl hydrolase family 71	polymer	385	43933.6	1	UniProt (D6D1V7) Pfam (PF16317)	Bacteroides xylanisolvens XB1A
2	B (B)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose	branched		342.3	1	In PDB BIRD (PRD_900111) GlyTouCan (G53402KW)		2alpha-alpha-mannobiose
3	C (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
4	D (A)	alpha-D-mannopyranose	non-polymer		180.2	1	Chemie (MAN)
5	E (A)	(2S,3S,4R,5R)-2,3,4-TRIHYDROXY-5-HYDROXYMETHYL-PIPERIDINE	non-polymer		163.2	1	Chemie (MNM)
6	F (A)	water	water		18.0	468	Chemie (HOH)

Sequence modifications

A: 17 - 380 (UniProt: D6D1V7)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
Ser 6	-	expression tag
Ser 7	-	expression tag
Gly 8	-	expression tag
Leu 9	-	expression tag
Val 10	-	expression tag
Pro 11	-	expression tag
Arg 12	-	expression tag
Gly 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
Met 16	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	43933.6
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	3
	Total formula weight	402.4
All*	Total formula weight	44678.3

*Water molecules are not included.