Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5LZV

Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1.

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	uL2	polymer	257	28088.9	1	UniProt (G1TT27) Pfam (PF00181) Pfam (PF03947)	Oryctolagus cuniculus (Rabbit)
2	B (B)	uL3	polymer	403	46108.0	1	UniProt (G1TL06) Pfam (PF00297)	Oryctolagus cuniculus (Rabbit)
3	C (C)	uL4	polymer	425	47727.6	1	UniProt (G1SVW5) Pfam (PF00573) Pfam (PF14374)	Oryctolagus cuniculus (Rabbit)
4	D (D)	uL18	polymer	297	34481.8	1	UniProt (G1SYJ6) UniProt (by SIFTS) (P19949)	Oryctolagus cuniculus (Rabbit)
5	E (E)	eL6	polymer	291	33028.3	1	UniProt (G1SKF7) Pfam (PF01159) Pfam (PF03868)	Oryctolagus cuniculus (Rabbit)
6	F (F)	uL30	polymer	247	29201.8	1	UniProt (G1TUB1) Pfam (PF08079) Pfam (PF00327)	Oryctolagus cuniculus (Rabbit)
7	G (G)	eL8	polymer	319	36221.5	1	UniProt (G1STW0) Pfam (PF01248)	Oryctolagus cuniculus (Rabbit)
8	H (H)	uL6	polymer	192	21871.4	1	UniProt (G1TX33) Pfam (PF00347) UniProt (by SIFTS) (G1SWI6)	Oryctolagus cuniculus (Rabbit)
9	I (I)	uL16	polymer	214	24643.1	1	UniProt (B7NZQ2) Pfam (PF00252)	Oryctolagus cuniculus (Rabbit)
10	J (J)	uL5	polymer	178	20288.5	1	UniProt (G1TUB8) Pfam (PF00281) Pfam (PF00673)	Oryctolagus cuniculus (Rabbit)
11	K (L)	eL13	polymer	211	24331.7	1		Oryctolagus cuniculus (Rabbit)
12	L (M)	eL14	polymer	218	23870.5	1	UniProt (G1SZ12) Pfam (PF01929)	Oryctolagus cuniculus (Rabbit)
13	M (N)	eL15	polymer	204	24207.3	1	UniProt (G1T0C1) Pfam (PF00827)	Oryctolagus cuniculus (Rabbit)
14	N (O)	uL13	polymer	203	23533.3	1		Oryctolagus cuniculus (Rabbit)
15	O (P)	uL22	polymer	184	21444.2	1	UniProt (G1TVT6) Pfam (PF00237) UniProt (by SIFTS) (G1SCJ6)	Oryctolagus cuniculus (Rabbit)
16	P (Q)	eL18	polymer	188	21699.7	1		Oryctolagus cuniculus (Rabbit)
17	Q (R)	eL19	polymer	196	23535.3	1		Oryctolagus cuniculus (Rabbit)
18	R (S)	eL20	polymer	176	20827.6	1		Oryctolagus cuniculus (Rabbit)
19	S (T)	eL21	polymer	160	18610.0	1	UniProt (G1SHQ2) Pfam (PF01157)	Oryctolagus cuniculus (Rabbit)
20	T (U)	eL22	polymer	128	14813.0	1		Oryctolagus cuniculus (Rabbit)
21	U (V)	uL14	polymer	140	14892.5	1	UniProt (G1T6D1) Pfam (PF00238)	Oryctolagus cuniculus (Rabbit)
22	V (W)	eL24	polymer	157	17825.1	1	UniProt (G1SE28) Pfam (PF01246)	Oryctolagus cuniculus (Rabbit)
23	W (X)	uL23	polymer	156	17768.2	1	UniProt (G1SE76) Pfam (PF03939) Pfam (PF00276)	Oryctolagus cuniculus (Rabbit)
24	X (Y)	uL24	polymer	145	17303.4	1	UniProt (G1SQH0) Pfam (PF16906) Pfam (PF00467)	Oryctolagus cuniculus (Rabbit)
25	Y (Z)	eL27	polymer	136	15835.8	1	UniProt (G1TXF6) Pfam (PF00467) Pfam (PF01777)	Oryctolagus cuniculus (Rabbit)
26	Z (a)	uL15	polymer	148	16620.6	1	UniProt (G1SNY0) Pfam (PF00828)	Oryctolagus cuniculus (Rabbit)
27	AA (b)	eL29	polymer	245	26708.7	1	UniProt (G1SGR6) Pfam (PF01779)	Oryctolagus cuniculus (Rabbit)
28	BA (c)	eL30	polymer	115	12807.1	1	UniProt (G1TDL2) Pfam (PF01248)	Oryctolagus cuniculus (Rabbit)
29	CA (d)	eL31	polymer	125	14494.9	1	UniProt (G1SHG0) Pfam (PF01198)	Oryctolagus cuniculus (Rabbit)
30	DA (e)	eL32	polymer	135	15898.9	1	UniProt (G1U437) Pfam (PF01655) UniProt (by SIFTS) (G1TUN8)	Oryctolagus cuniculus (Rabbit)
31	EA (f)	eL33	polymer	110	12580.8	1	UniProt (G1SF08) Pfam (PF01247)	Oryctolagus cuniculus (Rabbit)
32	FA (g)	eL34	polymer	117	13326.1	1	UniProt (G1U945) Pfam (PF01199) UniProt (by SIFTS) (G1TXG5)	Oryctolagus cuniculus (Rabbit)
33	GA (h)	uL29	polymer	123	14566.6	1	UniProt (G1SIT5) Pfam (PF00831)	Oryctolagus cuniculus (Rabbit)
34	HA (i)	eL36	polymer	105	12263.8	1	UniProt (G1TTQ5) Pfam (PF01158)	Oryctolagus cuniculus (Rabbit)
35	IA (j)	eL37	polymer	97	11111.0	1	UniProt (U3KPD5) Pfam (PF01907)	Oryctolagus cuniculus (Rabbit)
36	JA (k)	eL38	polymer	70	8238.9	1		Oryctolagus cuniculus (Rabbit)
37	KA (l)	eL39	polymer	51	6455.8	1	UniProt (G1TTN1) Pfam (PF00832)	Oryctolagus cuniculus (Rabbit)
38	LA (m)	eL40	polymer	102	11699.8	1		Oryctolagus cuniculus (Rabbit)
39	MA (n)	eL41	polymer	25	3473.5	1		Oryctolagus cuniculus (Rabbit)
40	NA (o)	eL42	polymer	106	12477.0	1	UniProt (G1U344) Pfam (PF00935) UniProt (by SIFTS) (G1T040)	Oryctolagus cuniculus (Rabbit)
41	OA (p)	eL43	polymer	92	10299.4	1	UniProt (G1SY53) Pfam (PF01780)	Oryctolagus cuniculus (Rabbit)
42	PA (r)	eL28	polymer	137	15783.6	1	UniProt (G1U7L1) Pfam (PF01778)	Oryctolagus cuniculus (Rabbit)
43	QA (s)	uL10	polymer	318	34380.5	1		Oryctolagus cuniculus (Rabbit)
44	RA (t)	uL11	polymer	165	17847.6	1	UniProt (G1SMR7) Pfam (PF03946) Pfam (PF00298)	Oryctolagus cuniculus (Rabbit)
45	SA (1)	Nascent chain	polymer	15	1788.0	1		Oryctolagus cuniculus (Rabbit)
46	TA (2)	P-site tRNA	polymer	76	24436.6	1		Oryctolagus cuniculus (Rabbit)
47	UA (3)	E-site tRNA	polymer	75	24102.3	1		Oryctolagus cuniculus (Rabbit)
48	VA (5)	28S ribosomal RNA	polymer	3543	1148115.4	1		Oryctolagus cuniculus (Rabbit)
49	WA (7)	5S ribosomal RNA	polymer	120	38691.9	1		Oryctolagus cuniculus (Rabbit)
50	XA (8)	5.8S ribosomal RNA	polymer	156	50143.6	1		Oryctolagus cuniculus (Rabbit)
51	YA (9)	18S ribosomal RNA	polymer	1869	602778.9	1		Oryctolagus cuniculus (Rabbit)
52	ZA (AA)	uS2	polymer	295	32958.0	1		Oryctolagus cuniculus (Rabbit)
53	AB (BB)	eS1	polymer	264	30002.1	1	UniProt (G1SS70) Pfam (PF01015)	Oryctolagus cuniculus (Rabbit)
54	BB (CC)	uS5	polymer	293	31327.4	1		Oryctolagus cuniculus (Rabbit)
55	CB (DD)	uS3	polymer	243	26715.3	1	UniProt (G1TNM3) Pfam (PF07650) Pfam (PF00189)	Oryctolagus cuniculus (Rabbit)
56	DB (EE)	eS4	polymer	263	29654.9	1		Oryctolagus cuniculus (Rabbit)
57	EB (FF)	uS7	polymer	204	22913.5	1	UniProt (G1TFM5) Pfam (PF00177)	Oryctolagus cuniculus (Rabbit)
58	FB (GG)	eS6	polymer	249	28751.9	1	UniProt (G1TM55) Pfam (PF01092)	Oryctolagus cuniculus (Rabbit)
59	GB (HH)	eS7	polymer	194	22168.9	1	UniProt (G1SVB0)	Oryctolagus cuniculus (Rabbit)
60	HB (II)	eS8	polymer	208	24263.4	1	UniProt (G1TJW1) Pfam (PF01201)	Oryctolagus cuniculus (Rabbit)
61	IB (JJ)	uS4	polymer	194	22641.6	1	UniProt (B7NZS8) Pfam (PF00163) Pfam (PF01479)	Oryctolagus cuniculus (Rabbit)
62	JB (KK)	eS10	polymer	165	18933.8	1	UniProt (G1TPV3) Pfam (PF03501)	Oryctolagus cuniculus (Rabbit)
63	KB (LL)	uS17	polymer	158	18468.8	1	UniProt (G1TRM4) Pfam (PF16205) Pfam (PF00366)	Oryctolagus cuniculus (Rabbit)
64	LB (MM)	eS12	polymer	132	14539.0	1	UniProt (G1SFR8) Pfam (PF01248)	Oryctolagus cuniculus (Rabbit)
65	MB (NN)	uS15	polymer	151	17259.4	1	UniProt (G1SP51) Pfam (PF08069) Pfam (PF00312)	Oryctolagus cuniculus (Rabbit)
66	NB (OO)	uS11	polymer	168	18134.0	1	UniProt (G1T1F0) Pfam (PF00411)	Oryctolagus cuniculus (Rabbit)
67	OB (PP)	uS19	polymer	145	17049.2	1	UniProt (G1U0Q2) Pfam (PF00203)	Oryctolagus cuniculus (Rabbit)
68	PB (QQ)	uS9	polymer	146	16477.4	1	UniProt (G1SGX4) Pfam (PF00380)	Oryctolagus cuniculus (Rabbit)
69	QB (RR)	eS17	polymer	135	15552.1	1	UniProt (G1TU13) Pfam (PF00833)	Oryctolagus cuniculus (Rabbit)
70	RB (SS)	uS13	polymer	152	17759.8	1	UniProt (G1TPG3) Pfam (PF00416)	Oryctolagus cuniculus (Rabbit)
71	SB (TT)	eS19	polymer	145	16106.6	1	UniProt (G1TN62) Pfam (PF01090)	Oryctolagus cuniculus (Rabbit)
72	TB (UU)	uS10	polymer	119	13398.8	1	UniProt (G1SIZ2) Pfam (PF00338)	Oryctolagus cuniculus (Rabbit)
73	UB (VV)	eS21	polymer	83	9124.4	1		Oryctolagus cuniculus (Rabbit)
74	VB (WW)	uS8	polymer	130	14865.6	1	UniProt (G1TG89) Pfam (PF00410)	Oryctolagus cuniculus (Rabbit)
75	WB (XX)	uS12	polymer	143	15844.7	1		Oryctolagus cuniculus (Rabbit)
76	XB (YY)	eS24	polymer	130	15107.9	1		Oryctolagus cuniculus (Rabbit)
77	YB (ZZ)	eS25	polymer	125	13776.2	1	UniProt (G1TDB3) Pfam (PF03297)	Oryctolagus cuniculus (Rabbit)
78	ZB (aa)	eS26	polymer	115	13047.5	1		Oryctolagus cuniculus (Rabbit)
79	AC (bb)	eS27	polymer	84	9480.2	1	UniProt (G1TZ76) Pfam (PF01667)	Oryctolagus cuniculus (Rabbit)
80	BC (cc)	eS28	polymer	69	7855.1	1	UniProt (G1TIB4) Pfam (PF01200)	Oryctolagus cuniculus (Rabbit)
81	CC (dd)	uS14	polymer	56	6690.8	1	UniProt (G1U7M4) Pfam (PF00253)	Oryctolagus cuniculus (Rabbit)
82	DC (ee)	eS30	polymer	133	14498.9	1	UniProt (G1T8A2) Pfam (PF00240) Pfam (PF04758)	Oryctolagus cuniculus (Rabbit)
83	EC (ff)	eS31	polymer	156	18004.0	1	UniProt (G1SK22) Pfam (PF00240) Pfam (PF01599)	Oryctolagus cuniculus (Rabbit)
84	FC (gg)	RACK1	polymer	317	35115.7	1	UniProt (G1SJB4) Pfam (PF00400)	Oryctolagus cuniculus (Rabbit)
85	GC (hh)	mRNA (UGA stop codon)	polymer	15	4753.9	1		Oryctolagus cuniculus
86	HC (ii)	eRF1(AAQ)	polymer	459	51667.6	1	UniProt (P62495) Pfam (PF03463) Pfam (PF03464) Pfam (PF03465)	Homo sapiens (Human)	eRF1,Protein Cl1,TB3-1
87	IC (jj)	ABCE1	polymer	599	67405.2	1	UniProt (G1SG72) Pfam (PF04068) Pfam (PF00037) Pfam (PF00005)	Oryctolagus cuniculus (Rabbit)
88	AD, AE, AF, AG, AH... (5, 9, hh, B, I...)	MAGNESIUM ION	non-polymer		24.3	300	Chemie (MG)
89	BO, CO, DO, SC, UC... (aa, dd, ff, g, j...)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
90	FO, GO (jj)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)

Sequence modifications

B: 1 - 403 (UniProt: G1TL06)

PDB	External Database	Details
Met 1	Gln 1	initiating methionine

C: 1 - 378 (UniProt: G1SVW5)
C: 379 - 425 (PDB: 5LZV)
D: 1 - 297 (UniProt: G1SYJ6)

PDB	External Database	Details
Met 1	Lys 1	initiating methionine

F: 1 - 247 (UniProt: G1TUB1)

PDB	External Database	Details
Arg 61	Gly 61	conflict
Arg 93	Gly 93	conflict
Met 131	Val 131	conflict
Ile 153	Val 153	conflict

G: 1 - 77 (PDB: 5LZV)
G: 78 - 319 (UniProt: G1STW0)

PDB	External Database	Details
Gly 244	Cys 167	conflict

a: 1 - 148 (UniProt: G1SNY0)

PDB	External Database	Details
Met 1	Gln 1	conflict

b: 1 - 226 (UniProt: G1SGR6)
b: 227 - 245 (PDB: 5LZV)
g: 1 - 116 (UniProt: G1U945)

PDB	External Database	Details
Lys 117	-	insertion

II: 1 - 208 (UniProt: G1TJW1)

PDB	External Database	Details
Arg 47	Gly 47	conflict

OO: -16 - -5 (PDB: 5LZV)
OO: -4 - 151 (UniProt: G1T1F0)
TT: 1 - 145 (UniProt: G1TN62)

PDB	External Database	Details
Gly 119	Trp 119	conflict

ii: 1 - 437 (UniProt: P62495)

PDB	External Database	Details
Met -21	-	initiating methionine
Arg -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Gly -11	-	expression tag
Met -10	-	expression tag
Ala -9	-	expression tag
Ser -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 183	Gly 183	conflict
Ala 184	Gly 184	conflict

Sequence viewer

Contents of the asymmetric unit

Polymers	Number of chains	87
Polymers	Total formula weight	3535563.1
Non-Polymers*	Number of molecules	310
Non-Polymers*	Total formula weight	8518.1
All*	Total formula weight	3544081.2

*Water molecules are not included.

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)