5LZQ

Crystal structure of Thermotoga maritima sodium pumping membrane integral pyrophosphatase in complex with imidodiphosphate and magnesium, and with bound sodium ion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	K(+)-stimulated pyrophosphate-energized sodium pump	polymer	735	78217.1	2	UniProt (Q9S5X0) Pfam (PF03030)	Thermotoga maritima MSB8	Membrane-bound sodium-translocating pyrophosphatase,Pyrophosphate-energized inorganic pyrophosphatase,Na(+)-PPase,Tm-PPase
2	C, D, E, F, G... (A, B)	MAGNESIUM ION	non-polymer		24.3	10	Chemie (MG)
3	H, J, K, Q (A, B)	IMIDODIPHOSPHORIC ACID	non-polymer		177.0	4	Chemie (2PN)
4	I, R (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	S, T (A, B)	water	water		18.0	6	Chemie (HOH)

Sequence modifications

A, B: 2 - 726 (UniProt: Q9S5X0)

PDB	External Database	Details
Met -8	-	initiating methionine
Arg -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Leu 353	Val 353	engineered mutation
Gly 395	Ser 395	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	156434.3
Non-Polymers*	Number of molecules	16
	Total formula weight	997.0
All*	Total formula weight	157431.3

*Water molecules are not included.