5LUC

Crystal structure of the D183N variant of human Alanine:Glyoxylate Aminotransferase major allele (AGT-Ma) at 1.8 Angstrom; internal aldimine with PLP in the active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, E, G, M...)	Serine--pyruvate aminotransferase	polymer	403	44280.2	8	UniProt (P21549) Pfam (PF00266)	Homo sapiens (Human)	SPT,Alanine--glyoxylate aminotransferase,AGT
2	I, K, M, O, Q... (A, B, E, G, M...)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	8	Chemie (PLP)
3	J, L, N, P, R... (A, B, E, G, M...)	2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		209.2	8	Chemie (BTB)
4	AA, BA, CA, DA, EA... (E, G, M, N, S...)	water	water		18.0	4154	Chemie (HOH)

Sequence modifications

A, B, E, G, M, N, S, T: 1 - 392 (UniProt: P21549)

PDB	External Database	Details
Met -10	-	initiating methionine
Gly -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Asn 183	Asp 183	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	354241.2
Non-Polymers*	Number of molecules	16
	Total formula weight	3651.1
All*	Total formula weight	357892.3

*Water molecules are not included.