5LKA
Crystal structure of haloalkane dehalogenase LinB 140A+143L+177W+211L mutant (LinB86) from Sphingobium japonicum UT26 at 1.3 A resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Haloalkane dehalogenase | polymer | 301 | 33766.2 | 1 | UniProt (D4Z2G1) Pfam (PF00561) | Sphingobium japonicum | |
| 2 | B (A) | THIOCYANATE ION | non-polymer | 58.1 | 1 | Chemie (SCN) | |||
| 3 | C (A) | water | water | 18.0 | 434 | Chemie (HOH) |
Sequence modifications
A: 2 - 296 (UniProt: D4Z2G1)
| PDB | External Database | Details |
|---|---|---|
| Ala 140 | Trp 140 | engineered mutation |
| Leu 143 | Phe 143 | engineered mutation |
| Trp 177 | Leu 177 | engineered mutation |
| Leu 211 | Ile 211 | engineered mutation |
| His 297 | - | expression tag |
| His 298 | - | expression tag |
| His 299 | - | expression tag |
| His 300 | - | expression tag |
| His 301 | - | expression tag |
| His 302 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 33766.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 58.1 | |
| All* | Total formula weight | 33824.3 |
