5LKA

Crystal structure of haloalkane dehalogenase LinB 140A+143L+177W+211L mutant (LinB86) from Sphingobium japonicum UT26 at 1.3 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Haloalkane dehalogenase	polymer	301	33766.2	1	UniProt (D4Z2G1) Pfam (PF00561) In PDB	Sphingobium japonicum
2	A	THIOCYANATE ION	non-polymer		58.1	1	Chemie (SCN)
3		water	water		18.0	434	Chemie (HOH)

Sequence modifications

A: 2 - 296 (UniProt: D4Z2G1)

PDB	External Database	Details
Ala 140	Trp 140	engineered mutation
Leu 143	Phe 143	engineered mutation
Trp 177	Leu 177	engineered mutation
Leu 211	Ile 211	engineered mutation
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33766.2
Non-Polymers*	Number of molecules	1
	Total formula weight	58.1
All*	Total formula weight	33824.3

*Water molecules are not included.