5LK4
Structure of the Red Fluorescent Protein mScarlet at pH 7.8
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | mScarlet | polymer | 219 | 24897.1 | 1 | UniProt (Q9U6Y8) Pfam (PF01353) | Discosoma sp. | DsRed |
| 2 | B (A) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 1 | Chemie (PG4) | |||
| 3 | C (A) | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 4 | D, E, F, G (A) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 4 | Chemie (PGE) | |||
| 5 | H (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 6 | I (A) | water | water | 18.0 | 196 | Chemie (HOH) |
Sequence modifications
A: 8 - 222 (UniProt: Q9U6Y8)
| PDB | External Database | Details |
|---|---|---|
| Ala 7 | - | expression tag |
| His 18 | Arg 17 | engineered mutation |
| Ser 22 | Thr 21 | engineered mutation |
| Met 23 | Val 22 | engineered mutation |
| Thr 42 | His 41 | engineered mutation |
| Gln 43 | Asn 42 | engineered mutation |
| Ala 45 | Val 44 | engineered mutation |
| Ser 58 | Ala 57 | engineered mutation |
| Nrq 67 | Gln 66 | chromophore |
| - | Tyr 67 | chromophore |
| - | Gly 68 | chromophore |
| Arg 71 | Lys 70 | engineered mutation |
| Ala 72 | Val 71 | engineered mutation |
| Phe 73 | Tyr 72 | engineered mutation |
| Thr 74 | Val 73 | engineered mutation |
| Tyr 84 | Lys 83 | engineered mutation |
| Gln 86 | Leu 85 | engineered mutation |
| Ala 105 | Val 104 | engineered mutation |
| Thr 112 | Ser 111 | engineered mutation |
| Glu 115 | Gln 114 | engineered mutation |
| Thr 118 | Cys 117 | engineered mutation |
| Leu 119 | Phe 118 | engineered mutation |
| Leu 125 | Phe 124 | engineered mutation |
| Arg 126 | Ile 125 | engineered mutation |
| Thr 128 | Val 127 | engineered mutation |
| Pro 132 | Ser 131 | engineered mutation |
| Glu 154 | Arg 153 | engineered mutation |
| Asp 161 | Glu 160 | engineered mutation |
| Lys 163 | His 162 | engineered mutation |
| Met 164 | Lys 163 | engineered mutation |
| Arg 167 | Lys 166 | engineered mutation |
| Arg 173 | His 172 | engineered mutation |
| Ala 176 | Val 175 | engineered mutation |
| Asp 177 | Glu 176 | engineered mutation |
| Thr 180 | Ser 179 | engineered mutation |
| Thr 181 | Ile 180 | engineered mutation |
| Lys 183 | Met 182 | engineered mutation |
| Met 190 | Leu 189 | engineered mutation |
| Ala 193 | Tyr 192 | engineered mutation |
| Asn 195 | Tyr 194 | engineered mutation |
| Arg 198 | Ser 197 | engineered mutation |
| Val 211 | Ile 210 | engineered mutation |
| Ser 218 | Thr 217 | engineered mutation |
| Ser 223 | - | expression tag |
| Thr 224 | - | expression tag |
| Gly 225 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 24897.1 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 996.0 | |
| All* | Total formula weight | 25893.1 |
