5LEM

Crystal structure of DARPin-DARPin rigid fusion, variant DD_Off7_11_3G124 in complex with Maltose-binding Protein and Green Fluorescent Protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DD_Off7_11_3G124	polymer	326	35288.9	1		synthetic construct
2	B (B)	Maltose-binding periplasmic protein	polymer	395	43172.6	1	UniProt (P0AEY0) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9)	Escherichia coli K-12	MBP,MMBP,Maltodextrin-binding protein
3	C (C)	Green fluorescent protein	polymer	249	28219.7	1	UniProt (P42212) Pfam (PF01353)	Aequorea victoria (Jellyfish)
4	D (A)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

B: 3 - 366 (UniProt: P0AEY0)

PDB	External Database	Details
Met -14	-	initiating methionine
Arg -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Met 0	-	expression tag
Lys 1	-	expression tag
Thr 2	-	expression tag
Asn 6	Lys 32	conflict
Gly 367	-	expression tag
Ser 368	-	expression tag
Gly 369	-	expression tag
Gly 370	-	expression tag
Thr 371	-	expression tag
Pro 372	-	expression tag
Gly 373	-	expression tag
Arg 374	-	expression tag
Pro 375	-	expression tag
Ala 376	-	expression tag
Ala 377	-	expression tag
Lys 378	-	expression tag
Leu 379	-	expression tag
Asn 380	-	expression tag

C: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Met -10	-	initiating methionine
Arg -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Cro	Ser 65	chromophore
Cro	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Arg 80	Gln 80	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	106681.2
All*	Total formula weight	106681.2

*Water molecules are not included.