5LCW

Cryo-EM structure of the Anaphase-promoting complex/Cyclosome, in complex with the Mitotic checkpoint complex (APC/C-MCC) at 4.2 angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Anaphase-promoting complex subunit 1	polymer	1944	216775.5	1	UniProt (Q9H1A4) Pfam (PF12859) Pfam (PF20518) Pfam (PF21282) Pfam (PF18122) In PDB	Homo sapiens (Human)	APC1,Cyclosome subunit 1,Mitotic checkpoint regulator,Testis-specific gene 24 protein
2	B	Anaphase-promoting complex subunit 11	polymer	84	9854.6	1	UniProt (Q9NYG5) Pfam (PF12861) In PDB	Homo sapiens (Human)	APC11,Cyclosome subunit 11,Hepatocellular carcinoma-associated RING finger protein
3	C, P	Cell division cycle protein 23 homolog	polymer	597	68921.0	2	UniProt (Q9UJX2) Pfam (PF04049) In PDB	Homo sapiens (Human)	Anaphase-promoting complex subunit 8,APC8,Cyclosome subunit 8
4	D	Anaphase-promoting complex subunit 15	polymer	121	14286.7	1	UniProt (P60006) Pfam (PF15243) In PDB	Homo sapiens (Human)	APC15
5	E	Anaphase-promoting complex subunit 16	polymer	110	11678.0	1	UniProt (Q96DE5) Pfam (PF17256) In PDB	Homo sapiens (Human)	APC16,Cyclosome subunit 16
6	F, H	Cell division cycle protein 27 homolog	polymer	824	91973.1	2	UniProt (P30260) Pfam (PF12895) Pfam (PF00515) In PDB	Homo sapiens (Human)	Anaphase-promoting complex subunit 3,APC3,CDC27 homolog,CDC27Hs,H-NUC
7	G, W	Anaphase-promoting complex subunit CDC26	polymer	85	9794.0	2	UniProt (Q8NHZ8) Pfam (PF10471) In PDB	Homo sapiens (Human)	Anaphase-promoting complex subunit 12,APC12,Cell division cycle protein 26 homolog
8	I	Anaphase-promoting complex subunit 4	polymer	808	92219.2	1	UniProt (Q9UJX5) Pfam (PF12894) Pfam (PF12896) In PDB	Homo sapiens (Human)	APC4,Cyclosome subunit 4
9	J, K	Cell division cycle protein 16 homolog	polymer	620	71747.5	2	UniProt (Q13042) Pfam (PF12895) Pfam (PF13424) In PDB	Homo sapiens (Human)	Anaphase-promoting complex subunit 6,APC6,CDC16 homolog,CDC16Hs,Cyclosome subunit 6
10	L	Anaphase-promoting complex subunit 10	polymer	185	21282.1	1	UniProt (Q9UM13) Pfam (PF03256) In PDB	Homo sapiens (Human)	APC10,Cyclosome subunit 10
11	M	Anaphase-promoting complex subunit 13	polymer	74	8528.3	1	UniProt (Q9BS18) Pfam (PF05839) In PDB	Homo sapiens (Human)	APC13,Cyclosome subunit 13
12	N	Anaphase-promoting complex subunit 2	polymer	822	93939.0	1	UniProt (Q9UJX6) Pfam (PF00888) Pfam (PF08672) In PDB	Homo sapiens (Human)	APC2,Cyclosome subunit 2
13	O	Anaphase-promoting complex subunit 5	polymer	755	85179.8	1	UniProt (Q9UJX4) Pfam (PF21371) Pfam (PF12862) In PDB	Homo sapiens (Human)	APC5,Cyclosome subunit 5
14	Q	Cell division cycle protein 20 homolog	polymer	374	41226.3	1	UniProt (Q12834) Pfam (PF12894) Pfam (PF00400) In PDB	Homo sapiens (Human)	p55CDC
15	R	Cell division cycle protein 20 homolog	polymer	499	54796.5	1	UniProt (Q12834) Pfam (PF12894) Pfam (PF00400) In PDB	Homo sapiens (Human)	p55CDC
16	S	Mitotic checkpoint serine/threonine-protein kinase BUB1 beta,Mitotic checkpoint serine/threonine-protein kinase BUB1 beta	polymer	342	39055.8	1	UniProt (O60566) Pfam (PF08311) In PDB	Homo sapiens (Human)	MAD3/BUB1-related protein kinase,hBUBR1,Mitotic checkpoint kinase MAD3L,Protein SSK1,MAD3/BUB1-related protein kinase,hBUBR1,Mitotic checkpoint kinase MAD3L,Protein SSK1
17	X, Y	Anaphase-promoting complex subunit 7	polymer	599	66929.4	2	UniProt (Q9UJX3) Pfam (PF13181) Pfam (PF13432) In PDB	Homo sapiens (Human)	APC7,Cyclosome subunit 7
18	Z	Mitotic spindle assembly checkpoint protein MAD2A	polymer	205	23533.9	1	UniProt (Q13257) Pfam (PF02301) In PDB	Homo sapiens (Human)	HsMAD2,Mitotic arrest deficient 2-like protein 1,MAD2-like protein 1

Sequence modifications

A: 1 - 1944 (UniProt: Q9H1A4)

PDB	External Database	Details
Phe 356	Pro 356	conflict

S: 1 - 310 (UniProt: O60566)
S: 529 - 560 (UniProt: O60566)

Contents of the asymmetric unit

Polymers	Number of chains	23
	Total formula weight	1331085.9
All*	Total formula weight	1331085.9

*Water molecules are not included.