5L9Y

Crystal structure of human heparanase, in complex with glucuronic acid configured aziridine probe JJB355

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Heparanase	polymer	389	43733.3	1	UniProt (Q9Y251) Pfam (PF03662) In PDB	Homo sapiens (Human)	Endo-glucoronidase,Heparanase-1,Hpa1
2	B	Heparanase	polymer	77	8542.8	1	UniProt (Q9Y251) In PDB	Homo sapiens (Human)	Endo-glucoronidase,Heparanase-1,Hpa1
3	C	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	1	In PDB GlyTouCan (G86851RC)
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5	A	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
6	A	(1~{S},2~{R},3~{S},4~{S},5~{S},6~{R})-2-(8-azidooctylamino)-3,4,5,6-tetrakis(oxidanyl)cyclohexane-1-carboxylic acid	non-polymer		360.4	1	Chemie (6S6)
7		water	water		18.0	227	Chemie (HOH)

Sequence modifications

A: 158 - 543 (UniProt: Q9Y251)

PDB	External Database	Details
Asp 155	-	expression tag
Pro 156	-	expression tag
Gly 157	-	expression tag
Arg 307	Lys 307	conflict

B: 36 - 109 (UniProt: Q9Y251)

PDB	External Database	Details
Asp 33	-	expression tag
Pro 34	-	expression tag
Gly 35	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52276.1
Branched	Number of molecules	1
	Total formula weight	367.3
Non-Polymers*	Number of molecules	6
	Total formula weight	989.0
All*	Total formula weight	53632.5

*Water molecules are not included.