5L8W

Structure of USP12-UB-PRG/UAF1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin carboxyl-terminal hydrolase 12	polymer	376	43742.6	1	UniProt (O75317) Pfam (PF00443)	Homo sapiens (Human)	Deubiquitinating enzyme 12,Ubiquitin thioesterase 12,Ubiquitin-hydrolyzing enzyme 1,Ubiquitin-specific-processing protease 12
2	B (B)	WD repeat-containing protein 48	polymer	580	65194.8	1	UniProt (Q8TAF3) Pfam (PF00400) Pfam (PF11816)	Homo sapiens (Human)	USP1-associated factor 1,WD repeat endosomal protein,p80
3	C (C)	Polyubiquitin-B	polymer	75	8519.8	1	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
4	D (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	E (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	F (C)	prop-2-en-1-amine	non-polymer		57.1	1	Chemie (AYE)	Homo sapiens (Human)
7	G, H, I (A, B, C)	water	water		18.0	18	Chemie (HOH)

Sequence modifications

A: 1 - 370 (UniProt: O75317)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

B: 9 - 580 (UniProt: Q8TAF3)

PDB	External Database	Details
Trp 1	-	expression tag
Ser 2	-	expression tag
His 3	-	expression tag
Pro 4	-	expression tag
Gln 5	-	expression tag
Phe 6	-	expression tag
Glu 7	-	expression tag
Lys 8	-	expression tag
Phe 369	Leu 369	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	117457.2
Non-Polymers*	Number of molecules	3
	Total formula weight	214.6
All*	Total formula weight	117671.8

*Water molecules are not included.