5L2K

Crystal structure of GEM42 TCR-CD1b-GMM complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	T-cell surface glycoprotein CD1b	polymer	300	33488.6	1	UniProt (P29016) Pfam (PF16497) Pfam (PF07654) In PDB	Homo sapiens (Human)
2	B	Beta-2-microglobulin	polymer	98	11617.0	1	UniProt (P61769) Pfam (PF07654) In PDB	Homo sapiens (Human)
3	D	GEM42 TCR alpha chain	polymer	204	22646.1	1		Homo sapiens
4	E	GEM42 TCR beta chain	polymer	243	27312.6	1		Homo sapiens
5	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
6	A, D, E	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
7	A, B	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
8	A, E	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
9	A	TETRACOSYL PALMITATE	non-polymer		593.1	1	Chemie (6UL)
10	D	CESIUM ION	non-polymer		132.9	1	Chemie (CS)
11	E	6-O-[(2R,3R)-3-hydroxy-2-tetradecyldocosanoyl]-alpha-L-idopyranose	non-polymer		715.1	1	Chemie (70E)
12		water	water		18.0	6	Chemie (HOH)

Sequence modifications

A: 2 - 278 (UniProt: P29016)

PDB	External Database	Details
Ala 160	Ile 178	engineered mutation
Gly 279	-	expression tag
Ser 280	-	expression tag
Gly 281	-	expression tag
Leu 282	-	expression tag
Asn 283	-	expression tag
Asp 284	-	expression tag
Ile 285	-	expression tag
Phe 286	-	expression tag
Glu 287	-	expression tag
Ala 288	-	expression tag
Gln 289	-	expression tag
Lys 290	-	expression tag
Ile 291	-	expression tag
Glu 292	-	expression tag
Trp 293	-	expression tag
His 294	-	expression tag
Glu 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	95064.2
Non-Polymers*	Number of molecules	17
	Total formula weight	2772.8
All*	Total formula weight	97837.0

*Water molecules are not included.