5KY3

mouse POFUT1 in complex with mouse Factor VII EGF1 mutant (T101A) and GDP-fucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	GDP-fucose protein O-fucosyltransferase 1	polymer	355	40457.3	1	UniProt (Q91ZW2) Pfam (PF10250)	Mus musculus (Mouse)	Peptide-O-fucosyltransferase 1,O-FucT-1
2	B (B)	Coagulation factor VII	polymer	40	4389.9	1	UniProt (P70375) Pfam (PF00008)	Mus musculus (Mouse)	Serum prothrombin conversion accelerator
3	C, D (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
4	E (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL) PubChem (753)
5	F (A)	GUANOSINE-5'-DIPHOSPHATE-BETA-L-FUCOPYRANOSE	non-polymer		589.3	1	Chemie (GFB) PubChem (135412609) PubChem (135432122) PubChem (135618130) PubChem (135820750) PubChem (135857542) PubChem (136771500) PubChem (137034801) PubChem (137286751) PubChem (154728870) PubChem (162951224) PubChem (171699380) PubChem (135398578) PubChem (135398655) PubChem (135398688) PubChem (135398715) PubChem (135402013) PubChem (135410864) PubChem (135442158) PubChem (135665286) PubChem (135815440) PubChem (135815442) PubChem (135829314) PubChem (136044550) PubChem (136153884) PubChem (136254584) PubChem (136597497) PubChem (136597502) PubChem (136649460) PubChem (136663939) PubChem (137105976) PubChem (137105977) PubChem (137105978) PubChem (137270783) PubChem (138454237) PubChem (169441079) PubChem (171439195)
6	G, H (A, B)	water	water		18.0	407	Chemie (HOH)

Sequence modifications

A: 33 - 384 (UniProt: Q91ZW2)

PDB	External Database	Details
Gly 30	-	expression tag
Ala 31	-	expression tag
Pro 32	-	expression tag

B: 87 - 126 (UniProt: P70375)

PDB	External Database	Details
Ala 101	Thr 101	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	44847.1
Non-Polymers*	Number of molecules	4
	Total formula weight	1123.9
All*	Total formula weight	45971.0

*Water molecules are not included.