5KWV

Crystal Structure of a Pantoate-beta-alanine Ligase from Neisseria gonorrhoeae with bound AMPPNP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Pantothenate synthetase	polymer	286	32047.5	2	UniProt (B4RKE7) Pfam (PF02569)	Neisseria gonorrhoeae	PS,Pantoate--beta-alanine ligase,Pantoate-activating enzyme
2	C, D (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
3	E, F (A, B)	water	water		18.0	157	Chemie (HOH)

Sequence modifications

A, B: 21 - 278 (UniProt: B4RKE7)

PDB	External Database	Details
Met -7	-	expression tag
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Met 1	-	expression tag
Gln 2	-	expression tag
Ile 3	-	expression tag
Ile 4	-	expression tag
His 5	-	expression tag
Thr 6	-	expression tag
Ile 7	-	expression tag
Arg 8	-	expression tag
Glu 9	-	expression tag
Leu 10	-	expression tag
Arg 11	-	expression tag
Thr 12	-	expression tag
Trp 13	-	expression tag
Arg 14	-	expression tag
Glu 15	-	expression tag
Asn 16	-	expression tag
Thr 17	-	expression tag
Gly 18	-	expression tag
Lys 19	-	expression tag
Val 20	-	expression tag
Gly 213	Ala 194	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	64094.9
Non-Polymers*	Number of molecules	2
	Total formula weight	1012.4
All*	Total formula weight	65107.3

*Water molecules are not included.