5KWQ

Two Tandem RRM Domains of FBP-Interacting Repressor (FIR), also Known as PUF60

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Poly(U)-binding-splicing factor PUF60	polymer	216	23408.5	2	UniProt (Q9UHX1) Pfam (PF00076) In PDB	Homo sapiens (Human)	60 kDa poly(U)-binding-splicing factor,FUSE-binding protein-interacting repressor,FBP-interacting repressor,Ro-binding protein 1,RoBP1,Siah-binding protein 1,Siah-BP1

Sequence modifications

A, B: 101 - 299 (UniProt: Q9UHX1)

PDB	External Database	Details
Gly 84	-	expression tag
Ser 85	-	expression tag
His 86	-	expression tag
Met 87	-	expression tag
Ala 88	-	expression tag
Ser 89	-	expression tag
Met 90	-	expression tag
Thr 91	-	expression tag
Gly 92	-	expression tag
Gly 93	-	expression tag
Gln 94	-	expression tag
Gln 95	-	expression tag
Met 96	-	expression tag
Gly 97	-	expression tag
Arg 98	-	expression tag
Gly 99	-	expression tag
Ser 100	-	expression tag
Gly 106	Arg 123	engineered mutation
Ser 112	Cys 129	engineered mutation
Ala 238	Cys 255	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	46817.1
All*	Total formula weight	46817.1

*Water molecules are not included.