5KWE
Thermostable mutant of halohydrin dehalogenase HheC - C153N
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Halohydrin dehalogenase | polymer | 271 | 29840.6 | 4 | UniProt (Q93D82) Pfam (PF13561) In PDB | Rhizobium radiobacter (Agrobacterium tumefaciens) | |
2 | A, B, C, D | CHLORIDE ION | non-polymer | 35.5 | 5 | Chemie (CL) | |||
3 | A, B, C, D | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) | |||
4 | water | water | 18.0 | 1196 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 3 - 254 (UniProt: Q93D82)
PDB | External Database | Details |
---|---|---|
Met -16 | - | initiating methionine |
Gly -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
His -9 | - | expression tag |
Ser -8 | - | expression tag |
Ser -7 | - | expression tag |
Gly -6 | - | expression tag |
Leu -5 | - | expression tag |
Val -4 | - | expression tag |
Pro -3 | - | expression tag |
Arg -2 | - | expression tag |
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
His 1 | - | expression tag |
Ala 2 | - | expression tag |
Asn 153 | Cys 153 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 119362.5 | |
Non-Polymers* | Number of molecules | 9 |
Total formula weight | 269.2 | |
All* | Total formula weight | 119631.8 |