5KWE

Thermostable mutant of halohydrin dehalogenase HheC - C153N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Halohydrin dehalogenase	polymer	271	29840.6	4	UniProt (Q93D82) Pfam (PF13561)	Rhizobium radiobacter (Agrobacterium tumefaciens)
2	E, G, I, J, L (A, B, C, D)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
3	F, H, K, M (A, B, C, D)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
4	N, O, P, Q (A, B, C, D)	water	water		18.0	1196	Chemie (HOH)

Sequence modifications

A, B, C, D: 3 - 254 (UniProt: Q93D82)

PDB	External Database	Details
Met -16	-	initiating methionine
Gly -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
His 1	-	expression tag
Ala 2	-	expression tag
Asn 153	Cys 153	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	119362.5
Non-Polymers*	Number of molecules	9
	Total formula weight	269.2
All*	Total formula weight	119631.8

*Water molecules are not included.