5KW1

Crystal Structure of the Two Tandem RRM Domains of PUF60 Bound to a Modified AdML Pre-mRNA 3' Splice Site Analogue

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Poly(U)-binding-splicing factor PUF60	polymer	216	23408.5	2	UniProt (Q9UHX1) Pfam (PF00076)	Homo sapiens (Human)	60 kDa poly(U)-binding-splicing factor,FUSE-binding protein-interacting repressor,FBP-interacting repressor,Ro-binding protein 1,RoBP1,Siah-binding protein 1,Siah-BP1
2	C (C)	DNA/RNA (30-MER)	polymer	30	8862.4	1		unidentified adenovirus
3	D (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	E, F (A, B)	water	water		18.0	98	Chemie (HOH)

Sequence modifications

A, B: 118 - 316 (UniProt: Q9UHX1)

PDB	External Database	Details
Gly 101	-	expression tag
Ser 102	-	expression tag
His 103	-	expression tag
Met 104	-	expression tag
Ala 105	-	expression tag
Ser 106	-	expression tag
Met 107	-	expression tag
Thr 108	-	expression tag
Gly 109	-	expression tag
Gly 110	-	expression tag
Gln 111	-	expression tag
Gln 112	-	expression tag
Met 113	-	expression tag
Gly 114	-	expression tag
Arg 115	-	expression tag
Gly 116	-	expression tag
Ser 117	-	expression tag
Gly 123	Arg 123	engineered mutation
Ser 129	Cys 129	engineered mutation
Ala 255	Cys 255	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	55679.5
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	55715.0

*Water molecules are not included.