5KSH

Crystal structure of penicillin-binding protein 2 from Neisseria gonorrhoeae containing an A501T mutation associated with cephalosporin resistance

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Penicillin-binding protein 2	polymer	540	59024.9	2	UniProt (P08149) Pfam (PF03717) Pfam (PF00905) In PDB	Neisseria gonorrhoeae	PBP-2
2	A, B	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	A, B	SULFATE ION	non-polymer		96.1	12	Chemie (SO4)
4		water	water		18.0	39	Chemie (HOH)

Sequence modifications

A, B: 44 - 581 (UniProt: P08149)

PDB	External Database	Details
Gly 42	-	expression tag
Ser 43	-	expression tag
Thr 501	Ala 501	engineered mutation
Leu 504	Phe 504	engineered mutation
Val 510	Ala 510	engineered mutation
Gly 516	Ala 516	engineered mutation
Ser 551	Pro 551	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	118049.7
Non-Polymers*	Number of molecules	15
	Total formula weight	1429.0
All*	Total formula weight	119478.8

*Water molecules are not included.