5KP3
Crystal Structure of Ketosteroid Isomerase from Pseudomonas putida (pKSI) bound to Equilenin; D40N, Y57(Cl-Y)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Steroid Delta-isomerase | polymer | 135 | 15048.6 | 2 | UniProt (P07445) Pfam (PF12680) | Pseudomonas putida | Delta(5)-3-ketosteroid isomerase |
| 2 | C, E (A, B) | EQUILENIN | non-polymer | 266.3 | 2 | Chemie (EQU) | |||
| 3 | D, F (A, B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 130 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 131 (UniProt: P07445)
| PDB | External Database | Details |
|---|---|---|
| Asn 40 | Asp 40 | engineered mutation |
| Leu 132 | - | expression tag |
| Val 133 | - | expression tag |
| Pro 134 | - | expression tag |
| Arg 135 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 30097.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 724.8 | |
| All* | Total formula weight | 30821.9 |
