5KND

Crystal structure of the Pi-bound V1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	V-type sodium ATPase catalytic subunit A	polymer	600	66347.8	3	UniProt (Q08636) Pfam (PF02874) Pfam (PF16886) Pfam (PF00006) Pfam (PF22919)	Enterococcus hirae ATCC 9790	Na(+)-translocating ATPase subunit A,V-type sodium pump catalytic subunit A
2	D, E, F (D, E, F)	V-type sodium ATPase subunit B	polymer	465	51720.9	3	UniProt (Q08637) Pfam (PF02874) Pfam (PF00006) Pfam (PF22919)	Enterococcus hirae ATCC 9790	Na(+)-translocating ATPase subunit B,V-type sodium pump subunit B
3	G (G)	V-type sodium ATPase subunit D	polymer	217	25119.0	1	UniProt (P43435) Pfam (PF01813)	Enterococcus hirae ATCC 9790	Na(+)-translocating ATPase subunit D,V-type sodium pump subunit D
4	H (H)	V-type sodium ATPase subunit NtpG (F)	polymer	115	12689.3	1	UniProt (P43455) Pfam (PF01990)	Enterococcus hirae ATCC 9790	V-type sodium ATPase subunit G,Na(+)-translocating ATPase subunit G,V-type sodium pump subunit G
5	I (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	J, L, N (C, F, G)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
7	K (C)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
8	M (F)	2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		282.3	1	Chemie (B3P)
9	O, P, Q, R, S... (A, B, C, D, E...)	water	water		18.0	64	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 593 (UniProt: Q08636)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

D, E, F: 1 - 458 (UniProt: Q08637)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

G: 1 - 210 (UniProt: P43435)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

H: 1 - 103 (UniProt: P43455)

PDB	External Database	Details
Ser 104	-	expression tag
Gly 105	-	expression tag
Pro 106	-	expression tag
Ser 107	-	expression tag
Ser 108	-	expression tag
Gly 109	-	expression tag
Glu 110	-	expression tag
Asn 111	-	expression tag
Leu 112	-	expression tag
Tyr 113	-	expression tag
Phe 114	-	expression tag
Gln 115	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	392014.5
Non-Polymers*	Number of molecules	6
	Total formula weight	677.9
All*	Total formula weight	392692.4

*Water molecules are not included.